羟基蛋氨酸锌对断奶仔猪镉损伤的修复作用

doi:10.11843/j.issn.0366-6964.2018.02.011

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (2): 318-326.doi: 10.11843/j.issn.0366-6964.2018.02.011

羟基蛋氨酸锌对断奶仔猪镉损伤的修复作用

刘粉粉^1,2,3, 倪姮佳^1,2,3*, 黄攀^2,3, 吴信^1,2,3, 张彬^1*, 张维军⁴, 姚亚军⁴, 印遇龙^1,2,3

1. 湖南农业大学动物科学技术学院, 长沙 410128;
2. 中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术中心, 农业部中南动物营养与饲料科学观测实验站, 长沙 410125;
3. 中国科学院大学, 北京 100049;
4. 长沙兴嘉生物工程股份有限公司, 长沙 410001

收稿日期:2017-05-09 出版日期:2018-02-23 发布日期:2018-02-23
通讯作者: 倪姮佳,副研究员,E-mail:nihengjia@isa.ac.cn;张彬,教授,E-mail:zhb8236@126.com
作者简介:刘粉粉(1991-),女,山东单县人,硕士,主要从事单胃动物营养的研究,E-mail:liuff910511@163.com
基金资助:
国家自然科学基金青年基金项目（31501965）；国家重点研发计划项目（2016YFD0501201；2016YFD0200900）；中国科学院前沿科学重点研究项目（QYZDY-SSW-SMC008）；湖南农业科技创新资金项目（2017YC03）

The Protective Effect of Methionine Hydroxy Analog Chelated Zinc on Cadmium Inducing Damage in Weaned Piglets

LIU Fen-fen^1,2,3, NI Heng-jia^1,2,3*, HUANG Pan^2,3, WU Xin^1,2,3, ZHANG Bin^1*, ZHANG Wei-jun⁴, YAO Ya-jun⁴, YIN Yu-long^1,2,3

1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central of Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China;
4. Xingjia Bio-Engineering Co., Ltd., Changsha 410001, China

Received:2017-05-09 Online:2018-02-23 Published:2018-02-23

摘要/Abstract

摘要：

旨在探讨日粮添加羟基蛋氨酸锌（Methionine hydroxy analog chelated zinc，MHZn）对镉（Cadmium，Cd）引起断奶仔猪脏器损伤的修复作用。本研究选用24头45日龄的二元阉公猪（长×大，体重（13.22±1.36） kg）。随机分为4个处理组：对照组（Con组）、含镉日粮（30 mg·kg^-1，Cd组）组、镉（30 mg·kg^-1）+低浓度羟基蛋氨酸锌（100 mg·kg^-1）日粮（LMHZn组）组、镉（30 mg·kg^-1）+高浓度羟基蛋氨酸锌（200 mg·kg^-1）日粮（HMHZn组）组。每组6个重复，每个重复1头猪，代谢笼单栏饲喂，试验期30 d。第31天清晨对仔猪进行前腔静脉采血，空腹称重、屠宰、采样，称量肝、脾、肾重量，计算仔猪平均日增重、日采食量、料重比、脏器系数；制作肝、肾、十二指肠、空肠、回肠组织切片；检测肝、肾中Cd和微量元素含量；测定血浆生化指标。结果显示：1）与Con组相比，Cd组仔猪的平均日增重显著降低，料重比显著升高（P<0.05）；十二指肠和空肠肠绒毛长度显著降低（P<0.05），十二指肠、空肠的隐窝深度显著加深（P<0.05），十二指肠、空肠绒毛长度/隐窝深度比显著降低（P<0.05）；导致肝、肾细胞损伤、变性；肝、肾中的镉含量显著升高（P<0.05）；血浆白蛋白含量显著降低（P<0.05）。2）当含镉日粮中添加MHZn时，与Cd组相比，仔猪日增重显著升高（P<0.05），料重比显著降低（P<0.05），并且LMHZn和HMHZn组与Con组无显著差异（P>0.05）；提高十二指肠和空肠绒毛长度（P<0.05），减少仔猪小肠绒毛损伤；HMHZn组肝脏系数显著降低（P<0.05），并接近Con组水平，表明MHZn可有效缓解镉导致的肝肿大。并且，MHZn可有效缓解镉引起的肝细胞颗粒变性和脂肪变性，减少肾小管上皮细胞的变性和损伤，减少淋巴细胞的增生。MHZn （200 mg·kg^-1）可显著减少镉在肝、肾中的蓄积（P<0.05），减少镉对血浆白蛋白和总蛋白的影响，表明MHZn对缓解肝、肾、消化道镉损伤有一定的修复作用。综上所述，MHZn有助于缓解并修复镉对小肠、肝、肾的损伤。

Abstract:

This study aimed to investigate the protective effect of methionine hydroxy analog chelated zinc (MHZn) against cadmium inducing organ damage in weaned piglets. 24 barrows (Landrace×Large white, 45 day of age, (13.22±1.36)kg BW) were randomly allotted to 4 dietary groups (6 replicates per group, one piglet per replicate per pen):basal diet (control group, Con group), basal diet+30 mg·kg^-1 Cd (Cd group), basal diet + 30 mg·kg^-1 Cd+100 mg·kg^-1 MHZn (LMHZn group), basal diet+30 mg·kg^-1 Cd + 200 mg·kg^-1 MHZn (HMHZn group). The trial lasted for 30 days. On the morning of day 31, all of the piglets were weighted and blood samples were collected, then, all of the piglets were slaughtered and sampled. Liver, spleen and kidney were weighted to calculate organ coefficients. Average daily gain (ADG), average daily feed intake (ADFI) and the ratio of feed/gain (F/G) were calculated. We also conducted the histopathology examination of liver, kidney, duodenum, jejunum and ileum, the contents of Cd and metal trace elements in liver and kidney were detected, and the biochemical parameters in plasma were analyzed. The results showed that:1) Compared with Con group, piglets in Cd group had lower ADG and higher F/G ratio (P<0.05); dietary Cd also significantly decreased the villus length of duodenum and jejunum (P<0.05), increased crypt depth of duodenum and jejunum(P<0.05), decreased the ratio of V/C (P<0.05), increased Cd concentrations in liver and kidney (P<0.05). Hepatocellular degeneration and renal tubular degeneration were observed in piglets of Cd group. The concentrations of albumin in plasma significantly decreased after Cd treatment (P<0.05). 2) When piglets were fed diet with Cd and MHZn together, the ADG increased and F/G ratio decreased compared with piglets fed diet with Cd alone (P<0.05), and the value of ADG and F/D in LMHZn and HMHZn groups were not significantly different to Con group (P>0.05). Compared with Cd treatment, MHZn increased the villus length of duodenum and jejunum(P<0.05), suggesting that it could prevent the damage of intestinal villus. The liver coefficient in HMHZn group significantly decreased compared with Cd group (P<0.05), and the value of liver coefficient in HMHZn group was not significantly different to Con group (P>0.05), suggesting that MHZn relived hepatomegaly effectively. Besides, MHZn relived the granular degeneration and steatosis in liver, reduced renal tubular degeneration and lymphocyte proliferation in kidney. Moreover, MHZn (200 mg·kg^-1) decreased Cd accumulation in liver and kidney compared with Cd group(P<0.05), reduced the impact of Cd on albumin and total protein in plasma, suggesting that MHZn was helpful in reliving the Cd inducing damage on liver, kidney and gut. In summary, MHZn has beneficial effect on reliving and repairing the damage of small intestine, liver and kidney caused by Cd.

中图分类号:

S828.5

刘粉粉, 倪姮佳, 黄攀, 吴信, 张彬, 张维军, 姚亚军, 印遇龙. 羟基蛋氨酸锌对断奶仔猪镉损伤的修复作用[J]. 畜牧兽医学报, 2018, 49(2): 318-326.

LIU Fen-fen, NI Heng-jia, HUANG Pan, WU Xin, ZHANG Bin, ZHANG Wei-jun, YAO Ya-jun, YIN Yu-long. The Protective Effect of Methionine Hydroxy Analog Chelated Zinc on Cadmium Inducing Damage in Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 318-326.

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羟基蛋氨酸锌对断奶仔猪镉损伤的修复作用

The Protective Effect of Methionine Hydroxy Analog Chelated Zinc on Cadmium Inducing Damage in Weaned Piglets

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