山羊UBA52基因组织表达规律及其与性早熟的关联分析

doi:10.11843/j.issn.0366-6964.2013.09.007

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (9): 1380-1385.doi: 10.11843/j.issn.0366-6964.2013.09.007

山羊UBA52基因组织表达规律及其与性早熟的关联分析

王耀武¹，王永娟²，储明星^1*，曹贵玲³，王金玉^2*，狄冉¹，刘秋月¹

（1. 中国农业科学院北京畜牧兽医研究所，农业部畜禽遗传资源与种质创新重点实验室，北京 100193；2. 扬州大学动物科学与技术学院，扬州 225009； 3. 聊城大学农学院, 聊城 252059）

收稿日期:2013-03-06 出版日期:2013-09-23 发布日期:2013-09-23
通讯作者: 储明星，研究员，E-mail：mxchu@263.net；王金玉，教授，E-mail:jywang@yzu.edu.cn
作者简介:王耀武(1988-)，男，河南濮阳人，硕士生，主要从事动物分子遗传学研究，Tel：010-62816001，E-mail：wangyaowu0@126.com
基金资助:
国家现代农业产业技术体系建设专项资金(CARS-39)；国家自然科学基金项目(30871773)

Tissue Expression of UBA52 Gene and Its Association with Sexual Precocity in Goats

WANG Yao-wu¹, WANG Yong-juan², CHU Ming-xing^1*, CAO Gui-ling³, WANG Jin-yu^2*, DI Ran¹, LIU Qiu-yue¹

(1. Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Animal Science and Technology, Yangzhou University, Yangzhou225009, China; 3. College of Agriculture, Liaocheng University, Liaocheng 252059, China)

Received:2013-03-06 Online:2013-09-23 Published:2013-09-23

摘要/Abstract

摘要：

为了探索UBA52基因表达规律与山羊性早熟的关系，本研究以性早熟品种济宁青山羊与性晚熟品种辽宁绒山羊为研究对象，分别应用RT-PCR与荧光定量PCR技术检测UBA52基因在这2种山羊不同发育阶段的组织表达情况和下丘脑、垂体、卵巢、子宫、肾上腺5种组织中的mRNA表达水平。结果显示，UBA52基因在2种山羊各发育阶段各组织中广泛表达，并在子宫中的表达量最高，同时济宁青山羊垂体中UBA52基因表达量在初情期时极显著低于幼年期（P<0.01），而辽宁绒山羊UBA52基因表达量在幼年期Ⅱ时极显著高于幼年期Ⅰ，初情期时则极显著低于幼年期Ⅱ（P<0.01）。本研究结果表明UBA52基因表达上调与山羊发情呈负相关，推测UBA52表达上调可能通过使得促卵泡素(FSH)和促黄体素(LH)的分泌量减少来抑制山羊初情期的发生。

Abstract:

In order to detect the association of UBA52 gene and sexual precocity in goats, female sexual precocious goat breed Jining Grey goat and female sexual late-maturing goat breed Liaoning Cashmere goat were selected. RT-PCR method was used to detect the expression profile of UBA52 gene in tissues at different developmental stages of the two breeds，and then real-time PCR technology was used to exam the mRNA expression level of UBA52 genes in hypothalamus, pituitary gland, ovary, uterus and adrenal gland. The result showed that UBA52 gene was widely expressed in the organizations at different developmental stages of the two breeds, and its expression level in the uterus was the highest.The results also showed that the expression level of UBA52 in pituitary gland in puberty was significantly lower than that in juvenile period of Jining Grey goat（P<0.01）, while the expression level in juvenile period Ⅱwas significantly higher than that in juvenile period Ⅰof Liaoning Cashmere goat, and its expression level in puberty was significantly lower than that in juvenile period Ⅱ（P<0.01）. The results indicated that the up-regulated expression of UBA52 gene was negatively correlated to goats estrus. So it was concluded that the up-regulation of UBA52 may reduce the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) to suppress the occurrence of goat puberty.

中图分类号:

S827
S813.3

王耀武，王永娟，储明星，曹贵玲，王金玉，狄冉，刘秋月. 山羊UBA52基因组织表达规律及其与性早熟的关联分析[J]. 畜牧兽医学报, 2013, 44(9): 1380-1385.

WANG Yao-wu, WANG Yong-juan, CHU Ming-xing, CAO Gui-ling, WANG Jin-yu, DI Ran, LIU Qiu-yue. Tissue Expression of UBA52 Gene and Its Association with Sexual Precocity in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1380-1385.

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山羊UBA52基因组织表达规律及其与性早熟的关联分析

Tissue Expression of UBA52 Gene and Its Association with Sexual Precocity in Goats

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