猪源新型甲型H1N1流感病毒的分离鉴定及遗传进化特点

畜牧兽医学报

猪源新型甲型H1N1流感病毒的分离鉴定及遗传进化特点

张红娜¹，苗增民²，李欣¹，王浩¹，周玉法⁴，夏咸柱³，柴同杰^1*

1. 山东农业大学动物科技学院，泰安 271018； 2.泰山医学院生物科学学院，泰安 271016；3.中国军事医学科学院军事兽医研究所，长春 130000； 4. 泰安市岱岳区畜牧兽医局，泰安 271000

收稿日期:2012-09-03 出版日期:2013-04-23 发布日期:2013-04-23
通讯作者: 柴同杰(1957-)，男，山东德州人，教授，博士生导师，主要从事环境微生物和分子细菌学研究，E-mail: chaitj117@163.com
作者简介:张红娜（1986-），女，河北石家庄人，硕士生，主要从事环境微生物方向研究，E-mail: zhanghongna186982@126.com
基金资助:
国家环境保护环境微生物利用与安全控制重点实验2011年开放基金(MARC2011D061)；国家科技支撑计划子课题（2010BAD04B000）

Isolation, Identification and Phylogenetic Analysis of a Novel Swine-originInfluenza A (H1N1) Virus

ZHANG Hong-na¹, MIAO Zeng-min², LI Xin¹, WANG Hao¹, ZHOU Yu-fa⁴, XIA Xian-zhu³, CHAI Tong-jie^1*

1. College of Animal Science and Veterinary Medicine， Shandong Agricultural University，Tai’an 271018 China； 2. School of Biologic Science, Taishan Medical University， Tai’an 271016，China； 3. Institute of Military Veterinary， Academy of Military Medical Sciences PLA, Changchun 130000， China; 4. Animal Husbandry Bureau of Daiyue Region, Tai’an 271000, China

Received:2012-09-03 Online:2013-04-23 Published:2013-04-23

摘要/Abstract

摘要：

本研究旨在了解猪源新型甲型H1N1流感病毒山东分离株的遗传进化特点。对山东地区出现的疑似H1N1流感病死猪进行病料采样，然后进行病毒的分离鉴定，并对分离病毒株（A/swine/Shandong/07/2011）的HA、NA、PB2、PB1、PA、NP、NS和M基因进行遗传进化分析。结果显示，该株病毒8个片段的核酸序列与A/H1N1(2009)对应序列的相似性都大于99%，并且该毒株HA蛋白的裂解位点和优先识别唾液酸α-2,6受体的位点与A/H1N1(2009)也高度一致，分别为PSIQSR↓GLFGAI和190D、225D。但是，与A/H1N1(2009)毒株的HA蛋白相比，受体结合位点处出现了重要的突变（Q226R）。该研究结果为进一步研究猪源新型甲型H1N1流感病毒的分子进化提供了重要信息。

Abstract:

The study was carried out to examine the genetic characteristics of a novel swineorigin influenza A (H1N1) virus isolated from Shandong province. Samples from suspected influenza-infected pigs were collected for viral isolation and identification, and then genetic evolution of HA，NA，PB2，PB1，PA，NP，NS and M of the isolate (A/swine/Shandong/07/2011) was analyzed and compared with the related influenza viruses. The results showed that sequences of 8 fragments of the isolated virus revealed >99% nucleotide identity with A/H1N1(2009) prototype strain, and its HA cleavage and receptor-binding sites were PSIQSR↓GLFGAI and 190D, 225 D, respectively, which were identifical with A/H1N1(2009) . However, compared with HA protein of A/H1N1(2009), mutation Q226R occurred in receptor binding site of the isolate. In conclusion, the results of this study provided significant information for further research on molecular evolution of novel swine-origin influenza A (H1N1) virus.

中图分类号:

S852.659.5

张红娜，苗增民，李欣，王浩，周玉法，夏咸柱，柴同杰. 猪源新型甲型H1N1流感病毒的分离鉴定及遗传进化特点[J]. 畜牧兽医学报, doi: .

ZHANG Hong-na, MIAO Zeng-min, LI Xin, WANG Hao, ZHOU Yu-fa, XIA Xian-zhu, CHAI Tong-jie. Isolation, Identification and Phylogenetic Analysis of a Novel Swine-originInfluenza A (H1N1) Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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