鲁中地区猪高热性疾病的病理变化及病原学调查研究

畜牧兽医学报

鲁中地区猪高热性疾病的病理变化及病原学调查研究

刘月月^1，2，3，吴家强^2,3，任庆海¹，张绍武⁴，吴好庭⁵，张玉玉^2，3，赵鹏伟^2，3，彭军^1，2，3，李俊^2，3，杜以军^2，3，王金宝^1，2，3，刘思当^1*

(1.山东农业大学动物科技学院，泰安 271018； 2.山东省农业科学院畜牧兽医研究所，济南 250100；3.山东省畜禽疫病防治与繁育重点实验室，济南 250100； 4. 诸城市畜牧兽医管理局，诸城 271000；5.中国兽医药品监察所，北京 100081）

收稿日期:2012-07-05 出版日期:2012-12-26 发布日期:2012-12-26
通讯作者: 刘思当（1961-），E-mail：liusid@sdau.edu.cn
作者简介:刘月月（1987-），女，山东济南人，硕士生，主要从事动物临床病理学研究，E-mail：xiaoyueniuniu@163.com
基金资助:
泰安市科技发展计划；山东省优秀中青年科学家科研奖励基金（BS2009NY010）

Pathological Changes and Etiological Study of the Swine High Fever Syndrome (SHFS) in Central Region of Shandong Province

LIU Yue-yue^1，2，3 ，WU Jia-qiang^2，3，REN Qing-hai¹，ZHANG Shao-wu⁴，WU Hao-ting⁵，ZHANG Yu-yu^2，3，ZHAO Peng-wei^2，3， PENG Jun^1，2，3， LI Jun^2，3， DU Yi-jun^2，3，WANG Jin-bao^1，2，3， LIU Si-dang^1*

（1.College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China; 2. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 3.Shandong Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, China; 4. Zhucheng Animal Husbandry and Veterinary Medicine Management Bureau, Zhucheng 271000, China; 5.China Institute of Veterinary Drug Control, Beijing 100081, China）

Received:2012-07-05 Online:2012-12-26 Published:2012-12-26

摘要/Abstract

摘要：

为研究鲁中地区猪高热性疾病的病理变化及主要发病原因，对2011年1月-2012年3月患高热性疾病的56个患病猪群109头病猪进行了临诊症状及剖检病变观察，并对发病猪群进行追踪调查，采集363份发病猪群的血清，用ELISA方法检测血清样品的CSFV、PRRSV及PCV2抗体，用RTPCR和PCR方法检测病料中这3种病原，并对病料进行病理组织学及免疫组化检测。结果显示：患高热性疾病的猪群均已免疫过CSF、部分免疫过PRRS弱毒活疫苗，但均未进行PCVD疫苗免疫；发病猪群的CSF、PRRS及PCV2均有较高的抗体阳性率；病猪以淋巴组织急性炎症、间质性肺炎、病毒性脑炎的病变为主，其病变率分别为92.3%、76.1%和66.1%；CSFV、PRRSV和PCV2的病原检出率分别为30.27%、66.97%、41.28%，其中CSFV与PRRSV、CSFV与PCV2、PRRSV与PCV2二重感染检出率分别为16.51%、6.42%、28.44%，三者共感染检出率为4.59%，8.26%的病料未检测到这3种病原，属其它病原感染所致。序列分析表明CSFV流行株正在向着远离猪瘟兔化弱毒疫苗的方向发展， PRRSV均为高致病性变异毒株， PCV2则为毒力较强的PCV2b型。CSFV、PRRSV、PCV2是目前导致鲁中地区猪高热性疾病的主要病原，其中以PRRSV变异强毒感染最为常见，共感染是导致重症型猪高热性疾病的主要原因，病例还多见支原体、副猪嗜血杆菌等细菌病继发感染的病变，故该区域猪高热性疾病为多病原混合感染所致。

Abstract:

In order to study the pathological changes and the etiological agent of the Swine High Fever Syndrome (SHFS) in central region of Shandong province, we diagnosed the clinical symptoms and the pathological changes of 109 cases in 56 pig farms and collected 363 serum samples from Jan. 2011 to Mar. 2012. Three viruses were detected on tissue samples by histopathological, immunohistochemical examination and RT-PCR or PCR, including classical swine fever virus (CSFV)，porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV-2). Serological tests were used for detecting prevalence of CSFV，PRRS, and PCV- 2 antibody by ELISA. The results showed that all pigs suffering from high fever had been vaccinated by Classical Swine Fever vaccines, part by Porcine Reproductive and Respiratory Syndrome attenuated live vaccines, but none by Circovirus vaccines. However, they had high positive rates of antibody against CSFV，PRRSV, and PCV-2. The pathological changes mainly included acute inflammation in lymphoid tissues, interstitial pneumonia and viral encephalitis, and the occurrences were respectively 92.3%, 76.1% and 66.1%.The pathogenetic rates of CSFV, PRRSV and PCV2 were respectively 30.27%, 66.97% and 41.28%. Co-infection rates of CSFV and PRRSV, CSFV and PCV2, PRRSV and PCV2 were respectively 16.51%, 6.42% and 28.44%. And the triple infection rate was 4.59%. In addition, other pathogenic infections were 8.26%. Sequence analysis showed that the epidemic strains of CSFV were developing towards being far away from the HCLV, PRRSV strains were highly pathogenic ones, and PCV2 strains were mainly the virulent Type-PCV2b. In conclusion, CSFV, PRRSV and PCV2 were the main pathogens which caused the Swine High Fever in central region of Shandong province, and the severe cases were mainly caused by co-infection. What’s more, mixed infection of Mycoplasma and Haemophilus parasuis used to occur in clinical cases. Therefore, the Swine High Fever in the region was due to multiple infection.

中图分类号:

S858.285.3

刘月月，吴家强，任庆海，张绍武，吴好庭，张玉玉，赵鹏伟，彭军，李俊，杜以军，王金宝，刘思当. 鲁中地区猪高热性疾病的病理变化及病原学调查研究[J]. 畜牧兽医学报, doi: .

LIU Yue-yue，WU Jia-qiang，REN Qing-hai，ZHANG Shao-wu，WU Hao-ting，ZHANG Yu-yu，ZHAO Peng-wei，PENG Jun，LI Jun，DU Yi-jun，WANG Jin-bao，LIU Si-dang. Pathological Changes and Etiological Study of the Swine High Fever Syndrome (SHFS) in Central Region of Shandong Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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