一株塞内卡病毒A型的分离鉴定及演化分析

doi:10.11843/j.issn.0366-6964.2025.06.046

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (6): 3041-3046.doi: 10.11843/j.issn.0366-6964.2025.06.046

• 研究简报 • 上一篇

一株塞内卡病毒A型的分离鉴定及演化分析

王淑娟(), 王东方, 马震原, 赵雪丽, 刘影, 杨海波, 柴茂, 王翠, 闫若潜*()

河南省动物疫病预防控制中心/河南省重大动物疫病监测预警及防控重点实验室, 郑州 450000

收稿日期:2024-08-09 出版日期:2025-06-23 发布日期:2025-06-25
通讯作者: 闫若潜 E-mail:snowangel517@163.com;yrq1688@126.com
作者简介:王淑娟(1985-)，女，河南鹤壁人，高级兽医师，硕士，主要从事动物疫病病原分子生物学及免疫学研究，E-mail: snowangel517@163.com
基金资助:
河南省重点研发与推广专项(232102110085; 232102110089; 232102111053); 河南省重点研发专项(231111111300); 河南省重大科技专项(221100110600); 河南省现代农业产业技术体系(HARS-22-12-T)

Isolation, Identification and Evolutionary Analysis of a Strain of Senecavirus A

WANG Shujuan(), WANG Dongfang, MA Zhenyuan, ZHAO Xueli, LIU Ying, YANG Haibo, CHAI Mao, WANG Cui, YAN Ruoqian*()

Henan Centre for Animal Disease Control & Prevention/Henan Key Laboratory of Animal Disease Monitoring & Early Warning & Control and Prevention, Zhengzhou 450000, China

Received:2024-08-09 Online:2025-06-23 Published:2025-06-25
Contact: YAN Ruoqian E-mail:snowangel517@163.com;yrq1688@126.com

摘要/Abstract

摘要：

河南某免疫过口蹄疫疫苗的猪场出现水泡样疫病，疑似感染A型塞内卡病毒(Senecavirus A, SVA)。本研究旨在分离鉴定该病毒，并进行遗传演化分析。将RT-PCR和FQ-PCR检测为核酸阳性的样品接种BHK-21细胞，并进行传代培养。通过间接免疫荧光试验、电镜观察、病毒全基因组序列测定等对该毒株进行鉴定，并进行演化分析。结果表明，该毒株为SVA，将其命名为HeNXX/swine/2017；电镜观察发现该病毒粒子呈典型的二十面体，直径30 nm左右；该毒株可在BHK-21细胞上高效增殖，病毒滴度最高为10^9.5TCID₅₀·mL^-1；基因组全长7 288 bp (不含PolyA)，与美国毒株同源性最高。本研究成功分离到一株SVA，为相关疾病的进一步研究提供基础。

关键词: A型塞内卡病毒, 分离, 鉴定, 演化分析

Abstract:

A blister-like disease was found on a pig farm which was immunized against foot-and-mouth disease in Henan province, it was suspected infected with Seneca virus type A. The purpose of this study was to isolate, identify the virus and analyze its genetic evolution. The samples' nucleic acid tested positive by RT-PCR and FQ-PCR were inoculated into BHK-21 cells and cultured by passage. The virus was identified by indirect immunofluorescence assay, electron microscope observation, whole genome sequence and evolutionary analysis. The results indicate that the strain was SVA, which was named HeNXX/swine/2017; electron microscopy showed that the virion was a typical icosahedron with a diameter of about 30nm; this SVA strain has a high breeding efficiency in BHK-21 cells, the highest virus titer was 10^9.5 TCID₅₀·mL^-1; the total genome length is 7 288 bp (without PolyA), and it has the highest homology with 2 USA strains. A strain of SVA was successfully isolated in this study, which will provide further study basis for the related disease.

Key words: Senecavirus A, isolation, identification, genetic evolution analysis

中图分类号:

S852.659.6

王淑娟, 王东方, 马震原, 赵雪丽, 刘影, 杨海波, 柴茂, 王翠, 闫若潜. 一株塞内卡病毒A型的分离鉴定及演化分析[J]. 畜牧兽医学报, 2025, 56(6): 3041-3046.

WANG Shujuan, WANG Dongfang, MA Zhenyuan, ZHAO Xueli, LIU Ying, YANG Haibo, CHAI Mao, WANG Cui, YAN Ruoqian. Isolation, Identification and Evolutionary Analysis of a Strain of Senecavirus A[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 3041-3046.

图/表 3

参考文献 12

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一株塞内卡病毒A型的分离鉴定及演化分析

Isolation, Identification and Evolutionary Analysis of a Strain of Senecavirus A

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