肉羊体外胚胎生产技术规模化应用中品种的影响

doi:10.11843/j.issn.0366-6964.2024.06.017

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (6): 2451-2459.doi: 10.11843/j.issn.0366-6964.2024.06.017

肉羊体外胚胎生产技术规模化应用中品种的影响

陈莹¹(), 陈大勇³(), 乌日嘎³, 仇春娟³, 范利宏³, 包梅荣³, 岳媛², 梁红艳¹, 张家新⁴, 田见晖², 安磊²^,*(), 汪立芹¹^,*()

1. 农业农村部草食家畜遗传育种与繁殖重点实验室，乌鲁木齐 830011
2. 中国农业大学，北京 100193
3. 内蒙古赛诺种羊科技有限公司，乌兰察布 011800
4. 内蒙古农业大学，呼和浩特 010018

收稿日期:2023-10-10 出版日期:2024-06-23 发布日期:2024-06-28
通讯作者: 安磊,汪立芹 E-mail:88370828@qq.com;Chendayong81@126.com;anleim@cau.edu.cn;wlq6304@126.com
作者简介:陈莹(1990-)，女，湖北钟祥人，博士生，主要从事动物遗传育种与繁殖研究，E-mail：88370828@qq.com
陈大勇(1981-)，男，内蒙古扎赉特旗人，硕士生，主要从事动物遗传育种与繁殖研究，E-mail：Chendayong81@126.com
第一联系人：
陈莹和陈大勇为同等贡献作者
基金资助:
新疆维吾尔自治区科技支疆项目(2022E02017);“科技创新2030”项目子课题(2022ZD040130305);自治区重大专项(2023A02011)

Influence of Meat Sheep Varieties on the Scale Application of in vitro Embryo Production Technology

Ying CHEN¹(), Dayong CHEN³(), Riga WU³, Chunjuan QIU³, Lihong FAN³, Meirong BAO³, Yuan YUE², Hongyan LIANG¹, Jiaxin ZHANG⁴, Jianhui TIAN², Lei AN²^,*(), Liqin WANG¹^,*()

1. Key Laboratory of Genetics Breeding and Reproduction of Grass Feeding Livestock of Ministry of Agriculture and Rural Affairs, Urumqi 830011, China
2. China Agricultural University, Beijing 100193, China
3. Inner Mongolia Sino Sheep Technology Co. Ltd., Ulanqab 011800, China
4. Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2023-10-10 Online:2024-06-23 Published:2024-06-28
Contact: Lei AN, Liqin WANG E-mail:88370828@qq.com;Chendayong81@126.com;anleim@cau.edu.cn;wlq6304@126.com

摘要/Abstract

摘要：

旨在分析品种因素对OPU-IVEP商业化快繁效率的影响。本研究以经品种鉴定的健康8月龄至3周岁黑头萨福克、黑头杜泊、德克赛尔肉用种母羊为试验供体，在内蒙古赛诺种羊科技有限公司，采用OPU技术获取卵母细胞并进行体外胚胎工厂化生产或经腹腔镜输精和手术冲胚获得体内胚胎，进行胚胎移植，记录卵母细胞回收、胚胎发育以及移植妊娠的相关数据，进行统计分析。比较不同品种在体外胚胎规模化生产中卵母细胞回收效率、卵母细胞体外受精和胚胎发育能力、胚胎移植妊娠和产羔效率等方面的差异；同时，还比较了不同品种体内、外胚胎生产技术的应用效果。结果表明：1)3个品种间卵母细胞回收率差异极显著(P＜0.01)，黑头杜泊回收效率最高；2)3个品种的体外受精卵裂率无显著差异(P＞0.05)，但德克赛尔羊的囊胚发育率极显著高于其他两个品种(P＜0.01)；3)无论体内胚胎还是体外胚胎生产移植，各品种间胚胎移植受胎率和平均产羔数均无显著差异(P＞0.05)，且各品种内，体内胚胎和体外胚胎移植的平均受胎率和产羔率均无显著差异(P＞0.05)。综上所述，在工厂化、规模化应用绵羊体外胚胎生产技术过程中，不同品种间卵泡发育、回收卵母细胞数、囊胚发育率等存在差异，平均卵泡数和平均回收卵母细胞最少的德克塞尔羊的囊胚发育率最高；移植后受胎和产羔情况在不同品种间无显著差异，体内胚胎和体外胚胎单次生产获得的后代数也无显著差异，但OPU-IVEP的供体利用率远大于MOET。

关键词: 品种, 肉羊, 体外胚胎生产, 胚胎移植, 规模化, 应用

Abstract:

The aim of this study was to explore the impact of breed factors on the efficiency of commercial rapid propagation of OPU-IVEP. This study used healthy 8-month-old to 3-year-old black headed Suffolk, black headed Dorper, and Texel meat breeding ewes identified by breed identification as donors. OPU technology was used to obtain oocytes at Inner Mongolia Saino Sheep Technology Co., Ltd. for in vitro embryo factory production or in vivo embryos obtained through laparoscopic insemination and surgical flushing. Embryo transfer was performed, and relevant data of oocyte recovery, embryo development, and transfer pregnancy were recorded for statistical analysis. The differences in oocyte recovery efficiency, oocyte in vitro fertilization and embryo development ability, embryo transfer pregnancy and lambing efficiency among different breeds in large-scale in vitro embryo production was compared; At the same time, the application effects of in vivo and in vitro embryo production technologies of different breeds were also compared. The results showed that: 1) there was a significant difference in oocyte recovery rates among the 3 breeds (P < 0.01), with the highest recovery efficiency observed in the black headed Dorper; 2) There was no significant difference in the in vitro fertilization and cleavage rates among the 3 breeds (P > 0.05), but the blastocyst development rate of Texel sheep was significantly higher than the other 2 breeds (P < 0.01); 3) There was no significant difference (P > 0.05) in the conception rate and average number of lambs produced by embryo transfer between different breeds, whether in vivo or in vitro, and there was no significant difference (P > 0.05) in the average conception rate and lambing rate of in vivo and in vitro embryo transfer in different breeds. In summary, in the process of OPU-IVEP industrial and large-scale application in sheep in vitro embryo production, there are differences in the efficiency among different breeds, such as follicle development, number of retrieved oocytes, and blastocyst development rate. Texel sheep, which has the lowest average number of follicles and average number of retrieved oocytes, has the highest blastocyst development rate; The conception and lambing situation after transplantation, which directly affects the efficiency of rapid reproduction, did not show significant differences among different varieties, and there was no significant difference in the number of offspring obtained from single production of in vivo and in vitro embryos. However, the donor utilization rate of OPU-IVEP is higher than that of MOET.

Key words: breed, meat sheep, in vitro embryo production, embryo transfer, scaling, application

中图分类号:

陈莹, 陈大勇, 乌日嘎, 仇春娟, 范利宏, 包梅荣, 岳媛, 梁红艳, 张家新, 田见晖, 安磊, 汪立芹. 肉羊体外胚胎生产技术规模化应用中品种的影响[J]. 畜牧兽医学报, 2024, 55(6): 2451-2459.

Ying CHEN, Dayong CHEN, Riga WU, Chunjuan QIU, Lihong FAN, Meirong BAO, Yuan YUE, Hongyan LIANG, Jiaxin ZHANG, Jianhui TIAN, Lei AN, Liqin WANG. Influence of Meat Sheep Varieties on the Scale Application of in vitro Embryo Production Technology[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2451-2459.

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品种 Breed	实施OPU总数/次 Total number of OPU implemented	卵泡数/个 No. of follicles	平均卵泡数/(个·次^-1) Average number of follicles	回收卵数/枚 No. of retrieved oocytes	平均回收卵数/(枚·次^-1) Average number of retrieved oocytes
黑头萨福克BS	977	16 368	17.12±0.65^ABa	16 835	17.44±0.54^A
黑头杜泊BD	686	12 723	18.87±0.75^Aa	14 257	21.19±0.77^B
德克赛尔DK	112	1 570	13.97±0.14^Bb	1 490	13.20±0.33^C
合计Total	1 775	30 661	17.49±0.52	32 582	18.54±0.66

品种 Breed	卵母细胞数/枚 No. of oocytes	卵裂胚胎数/枚 No. of cleavage embryos	卵裂率/% Cleavage rate	培养胚胎数/枚 No. of cultured embryos	囊胚数/枚 No. of blastocysts	囊胚率/% Blastocyst rate
黑头萨福克BS	22 043	16 596	76.77±3.15	9 727	5 478	57.06±1.31^A
黑头杜泊BD	15 947	11 097	72.94±2.76	8 464	4 359	53.54±2.55^A
德克赛尔DK	2 113	1 625	71.98±2.00	1 339	870	69.09±4.25^B
合计Total	40 103	29 318	73.11	19 530	10 707	54.82

品种 Breed	供体数/只次 No. of donors	移植胚胎数/只 No. of transferred embryos	移植受体数/只 No. of transplant recipients	怀孕母羊数/只 No. of pregnant ewes	移植受胎率/% Transplantation conception rate	产羔数/只 No. of lambs produced	平均产羔数/只 Average number of lambs per donor
黑头萨福克BS	1 532	9 264	5 230	3 790	75.20±1.33	5 058	3.33±0.19
黑头杜泊BD	605	2 525	1 813	1 244	70.39±2.80	1 903	3.15±0.36
德克赛尔DK	513	2 616	1 656	1 144	72.69±3.56	1 731	3.36±0.33
合计Total	2 650	1 4405	8 699	6 178	71.02	8 692	3.28

品种 Breed	供体数/只次 No. of donors	可用胚胎数/枚 No. of available embryos	平均胚胎数 Average number of embryos	移植受体数/只 No. of transplant recipient	怀孕母羊数/只 No. of pregnant ewes	移植受胎率/% Transplantation conception rate	产羔数/只 No. of lambs produced	平均产羔数/只 Average number of lambs per donor
黑头萨福克BS	515	1 860	4.17±1.86	1 289	997	71.64±5.66	1 116	2.52±1.11
黑头杜泊BD	4 825	25 946	5.71±0.44	16 683	11 890	71.59±2.33	15 573	3.43±0.27
德克赛尔DK	139	679	4.91±1.40	573	371	64.62±4.77	418	3.01±0.80
合计Total	5 479	28 485	5.20	18 545	13 258	71.49	17 107	3.12

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肉羊体外胚胎生产技术规模化应用中品种的影响

Influence of Meat Sheep Varieties on the Scale Application of in vitro Embryo Production Technology

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