冷诱导RNA结合蛋白参与小鼠H1N1甲型流感病毒感染的应答

doi:doi: 10.11843/j.issn.0366-6964.2016.08.016

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (8): 1652-1657.doi: doi: 10.11843/j.issn.0366-6964.2016.08.016

冷诱导RNA结合蛋白参与小鼠H1N1甲型流感病毒感染的应答

聂培婷^1，2，汤承¹，岳华^1*

（1.西南民族大学生命科学与技术学院，成都 610041；2.新疆天康畜牧生物技术股份有限公司制药事业部，乌鲁木齐 830011）

收稿日期:2015-12-28 出版日期:2016-08-23 发布日期:2016-08-23
通讯作者: 岳华，教授，E-mail：yhua900@163.com
作者简介:聂培婷（1989-），女，新疆霍城人，主要从事感染与免疫学研究，E-mail：540016852@qq.com
基金资助:
国家自然科学基金（31172307）；四川省教育厅创新团队（13TD0057）

Cold-inducible RNA Binding Protein of Mice Involved in Response to Influenza A Virus Infection in Mice

NIE Pei-ting ^1，2，TANG Cheng ¹，YUE Hua1^*

（1.College of Life Science and Technology，Southwest University for Nationalities，Chengdu 610041，China；
2.Xinjiang Tecon Animal Husbandry Bio-Technology CO.，LT，Urumqi 830011，China）

Received:2015-12-28 Online:2016-08-23 Published:2016-08-23

摘要/Abstract

摘要：

冷诱导RNA结合蛋白（CIRP）是哺乳动物间高度保守的多功能蛋白，但其在病毒感染中的作用尚不清楚，本研究旨在探讨CIRP对甲型流感病毒感染的应答。用H1N1甲型流感病毒PR-8株滴鼻接种成年BALB/c小鼠，分别于感染后24、48、72、96、120 h随机处死3只，用荧光定量RT-PCR和免疫组化法分别检测小鼠心、肝、脾和肺中CIRP基因和蛋白质的表达水平。基因定量检测结果显示，感染小鼠各被检器官中Cirp mRNA的转录量显著升高；免疫组化试验结果显示，CIRP在感染小鼠心肌细胞、肺泡上皮细胞、肺支气管上皮细胞和脾淋巴细胞的细胞质中表达量均有不同程度的升高。综上可见，CIRP参与机体对H1N1甲型流感病毒感染的应答，由于CIRP对炎性因子产生过程具有显著的调节作用，其在流感病毒感染过程中的作用值得深入研究。

Abstract:

Cold induced RNA binding protein (CIRP) is a highly conserved multifunctional protein among vertebrates，but its role in viral infection is unclear.The objective of this paper was to explore the response of CIRP to influenza virus infection.The BALB/c mice were intranasal inoculated with H1N1 influenza A virus.Three mice were randomly selected in each group and euthanized at 24，48，72，96，120 h post infection.The heart，liver，spleen and lung of each mouse were collected.Real-time RT-PCR and immunohistochemistry (IMH) were used to detect the transcription of Cirp mRNA and expression of CIRP in each organ.The results of Real-time RT-PCR exhibited that the Cirp mRNA significantly increased in all detected organs.The results of IMH test showed that CIRP protein was highly expressed in cytoplasm of myocardial cells，alveolar epithelial cells，bronchial epithelium cells and spleen lymphocytes.In summary，CIRP is involved in the response to influenza A virus infection，because CIRP can significantly regulate the production of inflammatory factors，the role of CIRP in influenza virus infection should be further studied.

中图分类号:

S852.23

聂培婷，汤承，岳华. 冷诱导RNA结合蛋白参与小鼠H1N1甲型流感病毒感染的应答[J]. 畜牧兽医学报, 2016, 47(8): 1652-1657.

NIE Pei-ting，TANG Cheng，YUE Hua. Cold-inducible RNA Binding Protein of Mice Involved in Response to Influenza A Virus Infection in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1652-1657.

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冷诱导RNA结合蛋白参与小鼠H1N1甲型流感病毒感染的应答

Cold-inducible RNA Binding Protein of Mice Involved in Response to Influenza A Virus Infection in Mice

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