两纯系鸡胃肠道黏膜细菌l6S rDNA的PCR-DGGE分析比较

畜牧兽医学报

两纯系鸡胃肠道黏膜细菌l6S rDNA的PCR-DGGE分析比较

张洁¹，容庭¹，刘志昌¹，王刚¹，林海丹³，贝锦龙²，陈庄^1*

（1. 广东省农业科学院畜牧研究所，畜禽育种国家重点实验室，广州 510640；2. 广东省农业科学院，广州 510640； 3. 广东省兽药与饲料监察总所，广州 510230）

收稿日期:2012-02-28 出版日期:2013-02-23 发布日期:2013-02-23
通讯作者: 陈庄(1963-), 男, 博士, 研究员, E-mail: Chenzh1963@vip.sina.com
作者简介:张洁(1984-), 女, 内蒙古人, 硕士，主要从事动物胃肠道菌群与营养研究，E-mail: zj0478@163.com
基金资助:
广东省农业科技攻关引导项目（2009B020307001）

Comparative Analysis of 16S rDNA of the Gastrointestinal Mucosa-associated Microbiota between Two Pure Lines Chicken by PCR-DGGE

ZHANG Jie¹, RONG Ting¹, LIU Zhi-chang¹, WANG Gang¹,LIN Hai-dan³,BEI Jin-long²,CHEN Zhuang^1*

(1. State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 2. Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 3. Guangdong Institute for Veterinary Drug and Feedstuffs Control, Guangzhou 510230, China)

Received:2012-02-28 Online:2013-02-23 Published:2013-02-23

摘要/Abstract

摘要：

以1日龄纯系的优质肉鸡A系(♂，A组)和惠阳胡须鸡(♂，B组)为实验动物，研究比较两纯系鸡1～28日龄胃肠道黏膜细菌微生物丛组成与动态变化特点。采用l6S rDNAV3 PCRDGGE的方法，分析第1、3、7、14、21、28日龄2组鸡胃肠道黏膜细菌基因组DNA。DGGE图谱中显示，28日龄2组鸡回肠泳道的条带数差异明显，嗉囊、腺胃及盲肠黏膜菌群结构的相似性均大于70%，十二指肠与空肠的相似性分别为40%和44%，回肠仅18%；21～28日龄2组鸡回肠黏膜菌群结构发生变化的程度不同，A组的动态变化值（%change）为70%，而B组仅11%。优质肉鸡A系和惠阳胡须鸡28日龄回肠黏膜菌群结构存在明显差异，这种差异可能是宿主遗传的影响所致，同时也是影响肉鸡生产性能的可能原因。

Abstract:

The experiment were conducted to analyze and compare the diversity and dynamic change of the gastrointestinal mucosa-associated microbiota (MAM) of high quality chicken line A (Pure Line♂, Group A) and Huiyang Beard chicken (Pure Line♂, Group B) during day 1-28. The V3 region of 16S rDNA of the mucosa-associated microbiota of two chicken pure lines were analyzed by Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) at day 1, 3, 7, 14, 21 and 28, respectively. DGGE profiles, based on the number of bands of the gel, indicated the MAM of ileum between the two groups were significantly different at day 28. The similarity of the MAM in craw, glandular stomach and cecum between the two groups were higher than 70%, while the similarity in duodenum, jejunum and ileum were 40%, 44% and 18% at day 28, respectively. The alterations of MAM of ileum were remarkably different between the two groups during day 21-28, with 70% change in group A and 11% change in group B. This diversity between the high quality chicken line A and Huiyang Beard chicken might be influenced by the host genetic and, at the same time, might be one of the possible cause for their different growth performance.

中图分类号:

S831.2

张洁，容庭，刘志昌，王刚，林海丹，贝锦龙，陈庄. 两纯系鸡胃肠道黏膜细菌l6S rDNA的PCR-DGGE分析比较[J]. 畜牧兽医学报, doi: .

ZHANG Jie, RONG Ting, LIU Zhi-chang, WANG Gang,LIN Hai-dan,BEI Jin-long,CHEN Zhuang. Comparative Analysis of 16S rDNA of the Gastrointestinal Mucosa-associated Microbiota between Two Pure Lines Chicken by PCR-DGGE[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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