北京油鸡羊膜上皮细胞的分离、培养及分化潜能的研究

畜牧兽医学报

北京油鸡羊膜上皮细胞的分离、培养及分化潜能的研究

高玉花，张文秀，吴芳春，关伟军^*，马月辉^*

( 中国农业科学院北京畜牧兽医研究所，北京 100193)

收稿日期:2011-11-19 出版日期:2013-02-23 发布日期:2013-02-23
通讯作者: 关伟军，教授，E-mail: weijuanguan301@gmail.com; 马月辉，研究员，E-mail: yuehui_ma@hotmail.com
作者简介:高玉花（1984-），女，硕士，河北廊坊人，主要从事动物细胞与分子生物学研究，Tel: 010-62896062，E-mail: yuhua_gao@yeah.net
基金资助:
国家科技支撑项目（2006BAD13B08；2008BADB2B01）；“863”课题基金资助项目（2006AA10Z198；2007AA10Z170）；转基因生物新品种培育科技重大专项（2008ZX08009-003）

Isolation, Culture and Differentiation Capacity Research of Beijing-You Chicken Amniotic Epithelial Cells (AEC)

GAO Yu-hua, ZHANG Wen-xiu, WU Fang-chun, GUAN Wei-jun^*, MA Yue-hui^*

(Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193，China)

Received:2011-11-19 Online:2013-02-23 Published:2013-02-23

摘要/Abstract

摘要：

旨在研究禽类胚胎羊膜上皮细胞的分离、培养、鉴定及向不同胚层细胞诱导分化等的生物学特性。本研究使用酶消化法从北京油鸡胎膜中分离羊膜上皮细胞，通过免疫荧光和RT-PCR方法鉴定羊膜上皮细胞标志物(Nanog、Oct-4、Sox-2、CK19和Vimentin)，并诱导其向成骨细胞、脂肪细胞和胰岛样细胞诱导分化，检测其多向分化潜能。结果表明，酶消化法获得的鸡羊膜上皮细胞不仅表达干细胞特有的标志，而且成功诱导分化为成骨细胞、脂肪细胞和胰岛样细胞。综上所述，北京油鸡羊膜上皮细胞在体外具有较强的自我更新能力及可塑性，可作为种子细胞进行濒危物种的保存，并为日后利用羊膜上皮细胞进行临床治疗提供一定的理论依据，同时也可为油鸡基因的改良提供模型。

Abstract:

This study aimed to research characteristics of poultry embryo amniotic epithelial cells (AECs) which were isolated, cultured, identified and induced to differentiate to different mesodermal cells. AECs were isolated from Beijing-You chicken fetal membranes using enzymes digestion, immunofluorescence and RT-PCR were used to identify Nanog, Oct-4, Sox-2, CK19 and Vimentin. The multi-lineage differentiation potential of AECs was investigated, which were induced to differentiate into osteoblasts, adipocytes and pancreatic-islet like cells cells. The result showed that chicken AECs not only expressed the special markers of stem cells, but were induced to differentiate into osteoblasts, adipocytes and pancreatic-islet like cells successfully. To sum up, the AECs isolated from Beijing-You chicken fetal membranes have a high reproductive capacity and plasticity in vitro, and can be used as seed cells for the preservation of endangered species and for clinical treatment. The results can provide the theoretical basis for clinical treatment using AECs.

中图分类号:

S831.1

高玉花，张文秀，吴芳春，关伟军，马月辉. 北京油鸡羊膜上皮细胞的分离、培养及分化潜能的研究[J]. 畜牧兽医学报, doi: .

GAO Yu-hua, ZHANG Wen-xiu, WU Fang-chun, GUAN Wei-jun, MA Yue-hui. Isolation, Culture and Differentiation Capacity Research of Beijing-You Chicken Amniotic Epithelial Cells (AEC)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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