出生后发育时期济宁青山羊乳腺中ERα和PR的分布及其mRNA表达

畜牧兽医学报

出生后发育时期济宁青山羊乳腺中ERα和PR的分布及其mRNA表达

尹玉涛，王树迎^*，刘宵，张德敏，侯衍猛，黄丽波

(山东农业大学动物科技学院动物生物技术与疾病防治重点实验室，泰安 271018)

收稿日期:2012-02-21 出版日期:2012-12-26 发布日期:2012-12-26
通讯作者: 王树迎，E-mail：sywang@sdau.edu.cn
作者简介:尹玉涛（1986-），女，山东威海人，硕士研究生，主要从事动物形态学和发育生物学研究，E-mail: yinyt@sina.cn
基金资助:
国家自然科学基金项目（30871832）；山东省自然科学基金（ZR2009DM027）；山东省科技攻关项目（2008GG30008018）

Localization of ERα and PR and Differential Expression of ERα mRNA and PR mRNA in Mammary Gland of Jining Gray Goat during Postnatal Development

YIN Yu-tao， WANG Shu-ying^*， LIU Xiao， ZHANG De-min， HOU Yan-meng， HUANG Li-bo

(Key Laboratory of Animal Biotechnology and Disease Control and Prevention , College of
Animal Science and Technology, Shandong Agricultural University, Tai’an 271018,China)

Received:2012-02-21 Online:2012-12-26 Published:2012-12-26

摘要/Abstract

摘要：

本研究旨在探讨济宁青山羊出生后发育过程中乳腺组织内雌激素受体α(ERα)和孕激素受体(PR)免疫阳性细胞的分布及平均光密度和mRNA分子水平的表达规律。应用SP免疫组化法和图像分析方法检测ERα和PR在济宁青山羊乳腺内的分布；以GAPDH为内参基因，qRT-PCR方法检测 ERα和PR mRNA的相对表达量。结果表明, ERα和PR阳性染色主要见于乳腺组织内的血管内皮细胞、基质细胞和腺管上皮细胞的细胞核中，偶见胞浆阳性着色，180日龄在腺泡上皮细胞亦可见PR阳性表达；ERα和PR平均光密度自出生~性成熟期呈现逐渐上升的趋势，且性成熟期显著高于出生当天和初情期(P<0.05)，但与180日龄相比无显著差异(P>0.05)。qRTPCR结果显示，ERα mRNA的表达量自出生~性成熟期呈现逐渐上升趋势，性成熟期最高，极显著高于出生当天和初情期(P<0.01)，180日龄略有下降。PR mRNA表达量自出生当天~性成熟期较低且组间差异不显著(P>0.05)，180日龄的表达量最高且极显著高于其他的3组(P<0.01)。济宁青山羊出生后发育过程中，ERα对腺管伸长和上皮分化以及PR对腺管分支和腺泡发育起促进作用。

Abstract:

The objective of the experiment was to study the localition and the average integrated optical density of ERα and PR immunopositive cells and the abundance of ERα mRNA and PR mRNA in mammary gland during postnatal development in Jining Gray goat. The SP immunohistochemical staining and Image analysis methods were used to localize ERα and PR immunoprotective(-ip) cells in mammary gland of Jining Gray goat. The relative expression of ERα mRNA and PR mRNA was analyzed by using the real time fouorescence quantitative PCR with GAPDH as an internal gene. ERα and PR had similar variations, immunoprotective staining was mainly localized in the nucleus of blood vessels endothelial cells, stromal cells and ductal epithelial cells of mammary gland,occasionally in cytoplasmic, PR-ip substance also found in alveolar epithelial cells in 180 days.The average optical density was gradually increased from birth day to sexual maturity, and it was significantly higher in sexual maturity than that in birth day and puberty (P<0.01) ,it was not different with that in 180 days (P>0.05).The results of qRT-PCR showed that the abundance of ERα mRNA was gradually increased from birth day to sexual maturity , and it was significantly higher in sexual maturity than that in birth day and puberty (P<0.01),there was a slightly decrease from sexual maturity to 180 days .While the abundance of PR mRNA from birth day to sexual maturity was lower and there was no significant difference during these groups. In 180 days,it was significantly higher than the other three groups (P<0.01). These results indicated that the estrogen receptors of alph and the progesterone receptors could promote the growth of glandular ,the hyperplasia of epithelial cells and the development of lobulo-alveolar during postnatal development in Jining Gray goat.

中图分类号:

S827
S813.3

尹玉涛，王树迎，刘宵，张德敏，侯衍猛，黄丽波. 出生后发育时期济宁青山羊乳腺中ERα和PR的分布及其mRNA表达[J]. 畜牧兽医学报, doi: .

YIN Yu-tao，WANG Shu-ying，LIU Xiao，ZHANG De-min，HOU Yan-meng，HUANG Li-bo. Localization of ERα and PR and Differential Expression of ERα mRNA and PR mRNA in Mammary Gland of Jining Gray Goat during Postnatal Development[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2012/V43/I12/1984

参考文献

［1］KUIPER G G, ENMARK E, PELTO HUIKKO M, et al. Cloning of a novel receptor expressed in rat prostate and ovary［J］. Proc Natl Acad Sci USA, 1996,93(12):5925-5930.

［2］ENMARK E, PELTO HUIKKO M, GRANDIEN K, et al. Human estrogen receptor beta-gene structure, chromosomal localization and expression pattern［J］.Clin Endocrinol Metab,1997, 2(12):4258-4265.

［3］CAPUCO A V, ELLIS S, WOOD D L, et al. Postnatal mammary ductal growth:threedimensional imaging of cell proliferation effects of estrogen treatment, and expression of steroid receptors in prepubertal calves［J］.Tissue Cell, 2002,34(3):143-154.

［4］SHAW J A, UDOKANG K，MOSQUERA J M, et al. Oestrogen receptors alpha and beta differ in normal human breast and breast carcinomas［J］.J Pathol,2002,198(4):450-457.

［5］SCHAMS D，KOHLENBERG S，AMSELGRUBER W, et al. Expression and localization of oestrogen and Progesterone receptors in the bovine mammary gland during development function and involution［J］.J Endocrinol,2003,177:305-317.

［6］BOCCHINFUSO W P, LINDZEY J K, HEWITT S C, et al.Induction of mammary gland development in estrogen receptor-alpha knockout mice.［J］.Endocrinology, 2000,141(8):2982-2994.

［7］COUSE J F, KORACH K S. Estrogen receptor null mice: What have we learned and where will they lead us［J］. Endocr Rev, 1999, 20(3):358-417.

［8］BRISKEN C, PARK S, VASS T, et al. A paracrine role for the epithelial progesterone receptor in mammary gland development［J］. PNAS,1998,95(9):5076-5081.

［9］SHYAMALA G. Progesterone signaling and mammary gland morphogenesis［J］. J Mamm Gland Biol Neoplasia,1999, 4(1): 89-104.

［10］蔡泉. 济宁青山羊品种资源调查［J］.中国草食动物，2008，28(6): 64.

［11］XU Y H, SATTLER G L, EDWARDS H, et al. Nuclear-labeling index analysis (NLIA), a software package used to perform accurate automation of cell nuclear-labeling index analysis on immunohistochemically stained rat liver samples ［J］ . Comp Methods Programs Biomed, 2000,63(1):55-70.

［12］SHYAMALA G, CHOU Y C, LOUIE S G, et al. Cellular expression of estrogen and progesterone receptors in mammary glands: regulation by hormones, development and aging［J］. J Ster Biochem Mol Biol, 2002,80(2):137-148.

［13］徐鹰妮,姜军，程鸿, 等，ERα和ERβ在不同类型乳腺组织的表达及其意义［J］.第三军医大学学报，2007,11(29):1093-1095.

［14］STERNS E E, ZEE B. Mammographic density changes in perimenopausal and postmenopausal women:is effect of hormone replacement therapy predietable? ［J］.Breast Cancer Res Treat, 2000,59(2):125-132.

［15］ZEPS N, BENTEL J M, JOHN M.Papadimitriou, et al. Murine progesterone receptor expression in proliferating mammary epithelial cells during normal pubertal development and adult estrous cycle: association with ERα and ERβ status［J］. J Histochem Cytochem， 1999,47(10): 1323-1330.

［16］HASLMA S Z, COUNTERMAN L J. Mammary stroma modulates hormonal responsiveness of mammary epithelium in vivo in the mouse［J］.Endocrinology,1991,129(4):2017-2023.

［17］HUMPHREYS R C, LYDON J O, MALLEY B W, et al.Mammary gland development is mediated by both stromal and epithelial progesterone receptors［J］.Mol Endocrinol,1997,11(6):801-811.

［18］COOKE P S, BUCHANAN D L, LUBAHN D B, et al.Mechanism of estrogen action:lessons from the estrogen receptor-alpha knockout mouse［J］.Biol Reprod,1998,59(3):470-475.

［19］ANDERSON E, CLARKE R B. Steroid receptors and cell cycle in normal mammary epithelium［J］. J Mamm Gland Biol Neoplasia, 2004,9(1): 3-13.

［20］SEHEDIN P, MITRENGA T, KAEEK M, et al. Estrous cyele regulation of mammary epithelial cell proliferation differentiation and death in the Sprague-Dawley rat:a model for investigating the role of estrous cycling in mammary carcinogenesis［J］. J Mamm Gland Biol Neoplasia, 2000,5(2):211-225.

［21］FEUERHAKE F, SIGG W, HOFTER E A, et al.Cell proliferation,apoptosis and expression of Bcl-2 and Bax in non-lactating human breast epithelium in relation to the menstrual cycle and reproductive history［J］.Breast Cancer Res Treat,2003,77(1):37-48.

[1]	严晓春, 习海娇, 李金泉, 王志英, 苏蕊. 内蒙古绒山羊绒毛性状基因组育种值估计准确性研究[J]. 畜牧兽医学报, 2024, 55(1): 120-128.
[2]	张晨俭, 李隐侠, 丁强, 刘伟佳, 王慧利, 何南, 吴家顺, 曹少先. CRISPR/Cas9技术高效制备山羊SOCS2基因编辑胚胎[J]. 畜牧兽医学报, 2024, 55(1): 129-141.
[3]	孟秋赤, 卢光玉, 陈定双, 林亚秋, 王瑞龙, 钟朝崧, 王永, 刘伟, 王友利, 李艳艳, 李志雄. 山羊GPR35基因表达特性分析及对皮下脂肪细胞分化作用的研究[J]. 畜牧兽医学报, 2023, 54(12): 4993-5007.
[4]	何志成, 秦晓晨, 吕永强, 李秀金, 边会龙, 罗军, 李聪. 萨能奶山羊体尺性状的多元逐步回归分析与生长曲线拟合[J]. 畜牧兽医学报, 2023, 54(12): 5301-5311.
[5]	张任豹, 周东辉, 周李生, 高霄霄, 柳楠, 贺建宁. 基于70 K SNP芯片分析济宁青山羊保种群体的遗传结构[J]. 畜牧兽医学报, 2023, 54(7): 2836-2847.
[6]	邵钺馨, 张新钰, 葛丽岩, 史怀平. 西农萨能奶山羊ATF4基因克隆及其功能初步研究[J]. 畜牧兽医学报, 2023, 54(6): 2353-2364.
[7]	刘杰, 许香萍, 邓铭, 邹娴, 江声伟, 刘德武, 柳广斌, 孙宝丽, 郭勇庆, 李耀坤. miR-144-5p靶向WNT5a对山羊卵巢颗粒细胞增殖、凋亡的影响[J]. 畜牧兽医学报, 2023, 54(6): 2421-2435.
[8]	许甜甜, 张彤彤, 王蒙, 王昕. 转录因子Foxq1通过WNT/β-catenin信号通路影响绒山羊毛囊干细胞增殖的研究[J]. 畜牧兽医学报, 2023, 54(6): 2653-2661.
[9]	曲比伍且, 李艳艳, 李鑫, 王永, 王友利, 刘伟, 朱江江, 林亚秋. 山羊APOA4基因抑制肌内脂肪细胞分化[J]. 畜牧兽医学报, 2023, 54(5): 1927-1938.
[10]	马克岩, 韩金涛, 白雅琴, 李讨讨, 马友记. 基于简化基因组测序的永登七山羊遗传多样性分析[J]. 畜牧兽医学报, 2023, 54(5): 1939-1950.
[11]	王丽, 郭雅茹, 张俊梅, 雷铭凯, 王振国, 张春香, 任有蛇. 1,25(OH)₂D₃及VDR敲除对山羊附睾头上皮细胞β防御素家族表达的影响[J]. 畜牧兽医学报, 2023, 54(5): 1990-2000.
[12]	朱俊儒, 韦唯, 裴党帅, 张芬鹊, 段瑜, 郭永峰, 江悦, 夏树立, 韩静, 侯金星, 安小鹏. PDCD4对奶山羊乳腺上皮细胞的凋亡及β-酪蛋白和TG合成的影响[J]. 畜牧兽医学报, 2023, 54(4): 1429-1440.
[13]	陈灿灿, 蒋婧, 孙晓燕, 任航行, 李杰. AGRP基因在山羊组织表达及其对黑色素生成的作用机制[J]. 畜牧兽医学报, 2023, 54(4): 1441-1451.
[14]	但一昕, 杨璐, 向华, 张焕容, 任玉鹏, 杨发龙, 何翃闳, 朱江江. BIRC5对山羊睾丸细胞周期、凋亡的影响[J]. 畜牧兽医学报, 2023, 54(4): 1511-1524.
[15]	叶峻宁, 邓铭, 薛慧雯, 柳广斌, 邹娴, 孙宝丽, 郭勇庆, 刘德武, 李耀坤. 影响山羊胎儿肌肉发育mRNA和lncRNA的鉴定与分析[J]. 畜牧兽医学报, 2023, 54(3): 989-1002.