Abstract: The Tibetan Plateau sheep is one of the ordinary domestic animal of QinghaiTibetan Plateau, while anatomical evidence of its special testicular microvasculature adaptability to the high altitude environment is still less. By using the methods of vascular proplasm and replica scanning electron microscopic, we studied the angioarchitecture of the Tibetan Plateau sheep spermatic cord and its functional relationship. The results were as follows: 1)The convoluted testicular artery, which was embedded in the network of the venous pampiniform plexus and gave off branches to supply the epididymis; 2)The collecting veins, which run parallelly and anastomose frequently and formed a “Binding Area of Pocket”, it was the initiation area of the pampiniform plexus; 3)The testicular artery and pampinifotm plexus could be divided into three grades correspondingly, the vasa vasorum deriving from branches of testicular artery to supply the wall of pampiniform plexus. From the above, the specific angioarchitectural characteristics of spermatic cord in Tibetan Plateau sheep are very different with other adult mammals.