Abstract: To evaluate the immune efficacy of the suicidal DNA vaccine against gooseorigin Newcastle disease, a suicidal DNA vaccine plasmid expressing HN gene of goose-origin Newcastle disease virus was constructed by inserting the HN gene fragment into the expression vector pSCA1, and named as pS-HN. The plasmid was then transfected into BHK-21 cells and the HN protein expression was demonstrated by indirect immunofluorescence assay. Animal immune experiment was conducted by inoculating goose with the pS-HN. The pc-HN, convention DNA vaccine expressing the same HN gene, pcDNA3.1(+) and pSCA1 were used as control. The results revealed that pS-HN was superior to pc-HN in inducing humoral immune response, cellular immune response and have better protective rate against goose-origin Newcastle disease virus. The study laid a foundation for evaluating the protective efficacy of pS-HN.