Abstract: This experiment was conducted to study the distribution, origin, transfer pattern and interaction of L1 and RTE in bovine and pig genomes. Using the software of RepeatMasker and Censor, the content and distribution of L1 and RTE in bovine and pig genomes were detected. The average content and length of L1 are 7.89% and 529 bp in bovine genome, and 10.64% and 608 bp in pig genome. The average content and length of RTE in bovine genome are 11.83% and 433 bp, and the pig genome does not contain RTE sequence. According to the nucleotide divergence, we divided the L1 in bovine and pig genomes into 6 families and 14 subfamilies. The content of L1 in bovine and pig genomes reduced with the increasing of the GC content of every chromosome, same as the content of RTE in bovine genome. The RTE in bovine genome originated from an unknown species by horizontal transfer and did not from the antique mammals vertically. All the content and category data of L1 and RTE indicated the appearance of adaptive evolution in L1, RTE and between L1 and RTE.