Abstract: Based on the report that the abnormal splice form deleted exon 4 of Lmbr1/C7orf2 associated with human Acheiropodia and the conservation of the alternative splices among species to some degree, the identification and expression analysis of chicken Lmbr1 similar transcript variant were conducted. Primers spanning chicken Lmbr1 exon 4 were designed to clone the alternative splice deleted exon 4（Lmbr1-β）of chicken Lmbr1-α. The primers spanning exon 4 could detect the expression of two different transcripts of chicken Lmbr1 at the same time. The tissue expression analysis in one day-old chicks and 5-6 day-old embryos of White Leghorn showed that two transcripts could be detected in all tissues, and comparing with chicken Lmbr1-α, Lmbr1-β was a minor transcript and its expression level was weak. Comparison between the Lmbr1 expression of E3-E11 in polydactyly Silkies and four tetradactyly White Leghorn showed that both Lmbr1-α and Lmbr1-β expressed in all detected chicken embryo tissues, Lmbr1-α was the main transcript product and Lmbr1-β was a minor transcript product in very weak expression. No significant tissue expression difference was identified between the two breeds. It showed that Lmbr1-α containing exon 4 was the main transcript of Lmbr1, which expressed in all detected tissues of the chicken and developing embryos, the development of polydactyly in Silky was not related with the expression of chicken Lmbr1-α and Lmbr1-β.