Abstract: CHOK1 cell was stablly transfected by pcDNA3.1(+)β6p eucaryon expression vector, then the expressing disposition of integrin αvβ6 was detected by indirect immunofluoresence assay (IFA). Tissue profile of porcine integrin αvβ6 in porcine tongue, lip, trachea and so on, was detected by indirect immunohistochemistry assay(IHA). The monoclonal antibody against porcine integrin β6 subunit was used as first antibody, two antibodies of goat against mice IgG labelled with fluorescein isothiocyanate(FITC) and horseradish peroxidase(HRP) respectively were used as secondary antibody. RESULTS: Viridis fluorescence was appeared in cell membrane and cytoplasm of CHOK1 cell stablly transfected by pcDNA3.1(+)β6p eucaryon expression vector, buffy idio-positive reaction was appeared in cell membrane and cytoplasm of porcine tongue, lip, trachea and so on, which indicates integrin αvβ6 receptor is generally expressed in porcine tissue cell, which lay a foundation for further understanding of the roles of integrin αvβ6 in FMDV infection.