猪流行性腹泻病毒S蛋白受体结合域的分析

畜牧兽医学报 ›› 2009, Vol. 40 ›› Issue (4): 528-532.doi:

猪流行性腹泻病毒S蛋白受体结合域的分析

孙东波^1，2，陈建飞¹，时洪艳¹，申识川¹，吕茂杰¹，范秀萍¹，陈洪岩¹，冯力¹*

1.中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室，哈尔滨 150001；2.黑龙江八一农垦大学动物科技学院，大庆 163319

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 冯力

Analysis of Receptor Binding Domain on the S Protein of Porcine Epidemic Diarrhea Virus

SUN Dong-bo^{1, 2}, CHEN Jian-fei¹, SHI Hong-yan¹, SHEN Shi-chuan¹,
LV Mao-jie¹, FAN Xiu-ping¹, CHEN Hong-yan¹, FENG Li¹*

1.Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China; 2. College of Animal Science and Technology,Heilongjiang August First Land Reclamation University, Daqing 163319, China

Received:1900-01-01 Revised:1900-01-01 Online:2009-04-25 Published:2009-04-25

摘要/Abstract

摘要： 以猪流行性腹泻病毒（PEDV）可溶性的猪氨基肽酶N受体为靶蛋白对PEDV S1基因特异性肽库进行3轮生物淘选，然后对30个淘选的噬菌体克隆进行测序。序列分析发现，2个噬菌体克隆展示无义氨基酸序列，其余28个噬菌体克隆展示的氨基酸序列位于PEDV S蛋白的第249-529位氨基酸区域，这个区域被命名为MRR。利用Bac-to-Bac杆状病毒表达系统对MRR基因进行真核表达。Western blot结果表明，表达的MRR重组蛋白能够与兔抗PEDV多克隆抗体反应。

Abstract: Porcine epidemic diarrhea virus (PEDV) S1 gene-targeted library was micropanned three times by using porcine aminopeptidase N (pAPN) as a cellular receptor of the PEDV, and thirty phage clones were sequenced using the specific primers. The result of sequence analysis indicated that polypeptides displayed by twenty-eight phage clones were focused on amino acids 249-529 (MER) of the S protein with the exception of two meaningless sequences. The MRR recombinant protein was expressed using Bac-to-Bac baculovirus expression kit. The result of Western blot demonstrated that MRR recombinant protein could react with rabbit antisera against PEDV.

孙东波;陈建飞;时洪艳;申识川;吕茂杰;范秀萍;陈洪岩;冯力. 猪流行性腹泻病毒S蛋白受体结合域的分析[J]. 畜牧兽医学报, 2009, 40(4): 528-532.

SUN Dong-bo;;CHEN Jian-fei;SHI Hong-yan;SHEN Shi-chuan;LV Mao-jie;FAN Xiu-ping;CHEN Hong-yan;FENG Li. Analysis of Receptor Binding Domain on the S Protein of Porcine Epidemic Diarrhea Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(4): 528-532.

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