Abstract: To investigate the effect of aluminum salt on ammonia production from poultry litter in commercial broiler houses, aluminum was added into hen dung at three different levels: 0, 2% and 4% (aluminum (g)/dung(g)). After aluminum salt treatment, the pH value, ammonia production, urease activity of treated dung and the content of ammonia nitrogen or total nitrogen in treated dung were measured respectively at indicated days. We found that ammonia released from dung and the pH value of dung decreased significantly after treatment compared with that of control dung. Furthermore, the urease activity in dung was significantly inhibited with a time course dependent manner after aluminum salt treatment (at 7 and 14 d ). In addition, the total nitrogen content, but not ammonia nitrogen content in dung was significantly decreased (P＜0.05) after 3 days′ treatment, especially in high level (4%) treatment group. These results suggested that pH value reduction, urease activity suppression and total nitrogen consumption induced by aluminum addition contributed to the decrease of ammonia production from dung. Taken together, these data showed that aluminum salt addition in poultry litter was an effective method to reduce ammonia volatilization, which may benefit to the control of ammonia production and environmental condition in hen house.