生长因子EGF、bFGF对猪孤雌胚体外发育的影响

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (1): 36-39.doi:

生长因子EGF、bFGF对猪孤雌胚体外发育的影响

刘吉;潘登科;张莉;牟玉莲;张勇;杨述林;王子荣;冯书堂

1．中国农业科学院北京畜牧兽医研究所，北京 100094；2．新疆农业大学动物科学学院，乌鲁木齐 830052

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-01-25 发布日期:2007-01-25

Effects of EGF or bFGF on the Development of Porcine Parthenogenetic Embryos in vitro

1Institute of Animal Science, Chinese Academy of Agricultural University, Beijing 100094, China； 2 Department of Animal Science，XinjiangAgricultural University,Urumqi 830052,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-01-25 Published:2007-01-25

摘要/Abstract

摘要： 在胚胎发育的不同阶段，分别在培养基中添加EGF和bFGF，研究EGF和bFGF对猪孤雌胚体外发育的作用。结果表明：在1细胞阶段添加EGF或bFGF，添加EGF能够显著提高孤雌胚的卵裂率（P<0.05）；2～4细胞阶段添加EGF和bFGF，添加EGF组和添加bFGF组的囊胚率都显著高于对照组（P<0.05），而添加bFGF组的囊胚率和囊胚细胞数都略高于对照组和添加EGF组。说明EGF和bFGF有利于猪孤雌胚的体外发育，而且，bFGF能够通过提高囊胚细胞数而提高猪孤雌胚的质量。

Abstract: EGF and bFGF were added into the culture medium in the different stages. We studied the effects of epidermal growth factor or bisac fibroblast growth factor on the development of porcine parthenogenetic embryos in vitro. The results as follows: The addition of EGF significantly enhanced the cleavage rate of porcine parthenogenetic embryos (P<0.05); The addition of EGF or bFGF significantly enhanced the rates of blastocysts formation of 2-4cell porcine parthenogenetic embryos (P<0.05); Additionally, bFGF had more numbers of blastocysts and higher rates of blastocysts formation than the EGF’s and the control. In conclusion, EGF and bFGF are propitious to the development of porcine parthenogenetic embryos in vitro. And bFGF increased the quality of blastocysts by increasing total cell numbers in porcine parthenogenetic embryos.

刘吉;潘登科;张莉;牟玉莲;张勇;杨述林;王子荣;冯书堂. 生长因子EGF、bFGF对猪孤雌胚体外发育的影响[J]. 畜牧兽医学报, 2007, 38(1): 36-39.

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