畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (9): 874-877.doi:

• 遗传繁育 • 上一篇    下一篇

牛基因组DNA两种提取方法的比较研究

程金华;朱化彬;王 栋;郝海生;杨 波
  

  1. 中国农业科学院北京畜牧兽医研究所,北京 100094
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-09-25 发布日期:2006-09-25

Comparative Study of Two Cattle Genomic DNA Extraction Methods

CHENG Jin-hua ;ZHU Hua-bin;WANG Dong;HAO Hai-sheng ;YANG Bo   

  1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-09-25 Published:2006-09-25

摘要: 研究哺乳动物基因组DNA的水煮抽提法,并将传统的酚仿抽提法和水煮法进行比较,水煮法即通过对细胞的煮沸和冷却,使细胞破裂、蛋白变性,从而获得用于PCR扩增的模板DNA的方法。通过电泳分析和PCR扩增检测了所提取基因组DNA的完整性、可行性。检测时采用3对引物对2种方法提取的DNA进行了PCR扩增,同时对冷冻保存的基因组DNA也进行了扩增检测。结果表明:水煮法提取基因组DNA是一种快速、简便、经济、高效的方法。

Abstract: The genomic DNA extraction method, cooking extraction method in water, was studied and the comparison with the traditional phenol and chloroform method was done.The cooking extraction method was the method from which genomic DNA was obtained when the cells were broken and proteins were denatured after the cells were cooked and cooled quickly. The feasibility and integrality of the extracted genomic DNA were detected through agrose gel electrophoresis and PCR amplification. During detection, the PCR amplification of the extracted genomic DNA was done with 3 pairs of primers and the genomic DNA was also amplified after preserved at -20 ℃.The analysis result indicated that cooking extraction method is a quick, simple, economic and efficient method for genomic DNA extraction.