Abstract: Oviductal epithelial cells (OECs) of mouse , goat and chick were cultured by different methods and examined percents of Vimentin positive cells by Vimentin monoclonal antibody, then on this basis in vitro development of mouse pronuclear embryos co-cultured with them were compared. The results are summarized as follows: Somatic cells grow well in culture system and almost all are epithelial cells. Percents of Vimentin positive cells of mouse , goat and chick OECs were 2.4±0.3, 1.3±0.3 and 1.8±0.4, respectively. The proportion of pronuclear embryos from Kunming mouse developing to blastocysts (61.7%，57.2% and 56.2%, respectively) did not differ when co-cultured with primary mouse, goat and chick OECs, suggesting that a good development of mouse embryos was also achieved when co-cultured with goat and chick OECs.