雌激素受体和低密度脂蛋白受体在动脉粥样硬化兔组织中的表达

畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (7): 690-694.doi:

雌激素受体和低密度脂蛋白受体在动脉粥样硬化兔组织中的表达

崔亚利; 赵德明; 梁万宁;宁章勇;杨建民;孙艳明

1. 中国农业大学动物医学院，北京 100094； 2. 河北农业大学动物科学技术学院，保定 071001; 3. 解放军第466医院，北京 100081

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-07-25 发布日期:2005-07-25

Expression of Estrogen Receptor and Low-density Lipoprotein Receptor in Tissues of Atherosclerotic Rabbit

CUI Ya-li;ZHAO De-ming;LIANG Wan-ning;NING Zhang-yong;YANG Jian-min;SUN Yan-ming

1. College of Veterinary Medicine, China Agricultural University, Beijing 100094，China;2. College of Animal Science and Technology, Agricultural University of Hebei,Baoding 071001,China； 3. The 466th Hospital of PLA, Beijing 100081,China

Received:1900-01-01 Revised:1900-01-01 Online:2005-07-25 Published:2005-07-25

摘要/Abstract

摘要： 用饲喂正常标准兔料的去势雌兔作为对照组，高胆固醇饮食法复制动脉粥样硬化模型。采用βactin做内参照的半定量RT-PCR方法，对2组去势雌兔心脏、肝脏组织内雌激素受体α和低密度脂蛋白受体mRNA的表达进行了研究。结果表明：2种受体在兔心脏和肝脏内均有表达；对照组，雌激素受体在心脏、肝脏内的相对表达量为0.43±0.12、0.39±0.20，低密度脂蛋白受体在心脏、肝脏内的相对表达量为0.27± 0.05、0.86±0.12，差异显著（P<0.01）；模型组，雌激素受体和低密度脂蛋白受体在心脏、肝脏内的相对表达量分别为0.29±0.03、0.27±0.06和0.04±0.01、0.17±0.02。

Abstract: In present study, atherosclerotic animal model were replicated with high cholesterol feedstuff , and the expressions of estrogen receptor α and low-density lipoprotein receptor mRNA in heart and liver were studied by half quantitive RT-PCR, the rabbits oophorectomized and fed with normal feedstuff were used as control．The results showed：the two sorts of receptors expressed in heart and liver；in control group, the relative expression of ERα mRNA in the heart and liver were 0.43±0.12, 0.39±0.20，the relative expression of low-density lipoprotein receptor mRNA in the heart and liver were 0.27±0.05, 0.86±0.12； The relative expression of estrogen receptor and low-density lipoprotein receptor mRNA in the heart and liver of model rabbits were 0.29±0.03, 0.27±0.06 and 0.04±0.01, 0.17±0.02 respectively．

崔亚利;赵德明;梁万宁;宁章勇;杨建民;孙艳明. 雌激素受体和低密度脂蛋白受体在动脉粥样硬化兔组织中的表达[J]. 畜牧兽医学报, 2005, 36(7): 690-694.

CUI Ya-li;ZHAO De-ming;LIANG Wan-ning;NING Zhang-yong;YANG Jian-min;SUN Yan-ming. Expression of Estrogen Receptor and Low-density Lipoprotein Receptor in Tissues of Atherosclerotic Rabbit[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2005, 36(7): 690-694.

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