Abstract: Three structure protein genes of strain TS of transmissible gastroenteritis virus(TGEV), sM(small membrane protein),M(integral membrane protein) and N(nucleocapsid protein) genes were obtained by RT-PCR using primers designed according to their published nucleotide sequence in GenBank, and they are 346 bp,932 bp,1 217 bp, respectively. After splicing, the respective lengths of the sM, M and N genes of strain TS of TGEV is 248 bp,789 bp and 1 149 bp and their predicted corresponding protein has 82, 262 and 382 amino acids(aa), respectively. The homology at the nucleotide levels for the sM gene between strain TS and Purdue,TFI,96-1933 was found to be 95.2%,92.7%,90.2% respectively, and that of the deduced aa sequence was 95.9%,97.2%,98.8% correspondingly. The nucleotide sequence homology of M gene of strains TS shared 95.2%,98.0%,99.6%,95.0% nucleotide identity and 97.0%,97.3%,98.5%,93.5% deduced amino acid with that of strains Purdue, TFI,TGEV H,96-1933. The nucleotide sequence homology of N gene between strain TS and Purdue, TFI, FS722/70, Korea,TO14,TGEV H, 96-1933 is 98.1%,97.7%,99.0%,98.3%,99.2%,99.0%,95.9% and putative amino acid homology is 97.9%,98.4%,99.0%,97.7%,99.5%,96.2%,96.6%, respectively. Sequence analysis indicated that sM and N genotypes of TGEV are conservative, there are two kinds sub-genes of TGEV in China and their origins are probably from abroad.