一株携带耐药基因blaCTX-M-27沙门菌P1-CTX噬菌体-质粒的分离及生物学特性分析

doi:10.11843/j.issn.0366-6964.2025.07.043

摘要/Abstract

摘要：

P1噬菌体-质粒作为肠杆菌科细菌抗生素耐药基因的载体受到人们的关注，本研究旨在探究携带抗生素耐药基因的P1噬菌体-质粒是否具有诱导和进一步转导等生物学特性问题。对沙门菌中携带耐第三代头孢菌素类耐药基因bla_CTX-M-27的P1噬菌体-质粒(命名为P1-CTX)的生物学特性进行研究，包括噬菌体效价、最佳感染复数、一步生长曲线、温度和酸碱稳定性等。结果显示，P1-CTX噬菌体-质粒可诱导产生有裂解能力的完整噬菌体，效价为10⁶ PFU·mL^-1，最佳感染复数为0.01；P1-CTX噬菌体在低于50 ℃环境下活力较好，耐强碱而不耐强酸。可感染沙门菌和大肠埃希菌，且在宿主菌中不产生适应代价，稳定性较好。综上表明，P1-CTX噬菌体-质粒具有正常的噬菌体生物学特性，可携带耐药基因通过转导的方式水平传播，且稳定性好。

关键词: 沙门菌, P1噬菌体-质粒, 抗生素耐药基因, 生物学特性

Abstract:

P1 phage-plasmid has garnered much attention as a carrier of antibiotic resistance genes in Enterobacteriaceae. This experiment was conducted to study the P1 phage-plasmid carrying antibiotic resistance genes whether have biological characteristics such as induction and further transduction. The biological characteristics of P1-CTX phage-plasmid carrying the third-generation cephalosporin resistance gene bla_CTX-M-27 in Salmonella were studied, including phage titer, optimal multiplicity of infection, one-step growth curve, temperature and acid-alkali stability. The results showed that P1-CTX phage-plasmid could induce complete phage with cleavage ability, the titer was 10⁶ PFU·mL^-1, and the optimal multiplicity of infection was 0.01. P1-CTX phage had good activity below 50 ℃ and could tolerate strong alkali but not strong acid. It can be infected with Salmonella and Escherichia coli. It has no effect on host growth and is very stable. In conclusion, the above results indicated that P1-CTX phage-plasmid had normal phage biological characteristics and could carry drug resistance genes through transduction and spread horizontally with good stability.

Key words: Salmonella, P1 phage-plasmid, antibiotic resistance genes, biological characteristics

中图分类号:

S852.6

赵秋云, 沈焕钧, 宋厚辉, 蒋红霞, 许成钢. 一株携带耐药基因bla_CTX-M-27沙门菌P1-CTX噬菌体-质粒的分离及生物学特性分析[J]. 畜牧兽医学报, 2025, 56(7): 3519-3527.

ZHAO Qiuyun, SHEN Huanjun, SONG Houhui, JIANG Hongxia, XU Chenggang. Isolation and Characterization of Salmonella Phage P1-CTX Carrying bla_CTX-M-27[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3519-3527.

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效价/(PFU·mL^-1) Titer	受体菌浓度/(CFU·mL^-1) Acceptor concentration	感染复数 Multiplicity of infection	3 h后效价/(PFU·mL^-1) Titer after 3 h
10²	10⁸	10^-6	1.32×10²
10³	10⁸	10^-5	1.65×10³
10⁴	10⁸	10^-4	2.05×10⁶
10⁵	10⁸	10^-3	1.59×10⁷
10⁶	10⁸	10^-2	1.97×10⁸
10⁷	10⁸	10^-1	1.03×10⁸
10⁸	10⁸	1	1.11×10⁸
10⁹	10⁸	10	1.17×10⁸

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