单细胞转录组测序技术在家养动物中的应用

doi:10.11843/j.issn.0366-6964.2024.08.002

摘要/Abstract

摘要：

单细胞转录组测序技术(single cell RNA sequencing, scRNA-seq)能够以高精度分辨率鉴定单个细胞类型和细胞状态，打破普通转录组测序(bulk RNA sequencing, RNA-seq)无法探究目标细胞具体表达特征的困境，在各个领域的探索中起到重要作用，目前广泛应用于人类及小鼠发育生物学、肿瘤学、免疫学、复杂疾病、肠道微生物组及临床应用等诸多领域。近年来，scRNA-seq在畜牧业领域也开展了一些开创性的研究并获得了一系列的成果，主要集中在动物繁殖性能、胚胎发育、关键性状解析等方面，但相较于在人类上的应用还较为薄弱，在其他领域的应用也仍有待深入。本文主要综述了scRNA-seq的工作流程及其在家养动物中应用的研究进展，以期为提高scRNA-seq分析效率以及在家养动物中的创新应用提供参考。

关键词: 单细胞, 转录组测序, 家养动物

Abstract:

Single-cell transcriptome sequencing technology(scRNA-seq) can identify individual cell types and cell states with high-precision resolution, breaking the dilemma that ordinary transcriptome sequencing unable to probe the specific expression characteristics of target cells, and playing an important role in the exploration of various fields, which is now widely used in many fields, such as human and mouse developmental biology, oncology, immunology, complex diseases, intestinal microbiome and clinical applications. In recent years, scRNA-seq has also carried out some pioneering research in the field of animal husbandry, mainly focusing on animal reproductive performance, embryonic development, analysis of key traits, etc. However, the application of scRNA-seq in other fields remains to be in-depth compared to its application in humans. In this paper, workflow of scRNA-seq and its application in domesticated animals is reviewed, intending to provide a reference for improving the efficiency of scRNA-seq analysis and its innovative application in domesticated animals.

Key words: single-cell, transcriptome sequencing, domesticated animals

中图分类号:

S813.1

张肖旭, 李昊, 冯平捷, 杨豪, 李新月, 吕冉, 潘章源, 储明星. 单细胞转录组测序技术在家养动物中的应用[J]. 畜牧兽医学报, 2024, 55(8): 3276-3287.

Xiaoxu ZHANG, Hao LI, Pingjie FENG, Hao YANG, Xinyue LI, Ran LÜ, Zhangyuan PAN, Mingxing CHU. Application of Single-Cell Transcriptome Sequencing Technology in Domesticated Animals[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3276-3287.

图/表 1

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技术/工具 Technology/tools	应用 Application	参考文献 References
scRNA-seq+细胞示踪技术 scRNA-seq+cellular tracer technology	肝细胞移植后状态的变化和分区特征研究	[77]
RIBOmap	小鼠脑组织中细胞类型特异性和脑区特异性翻译调控研究	[78]
scRNA-seq+成像质谱分析scRNA-seq+IMS	活化的恒定自然杀伤T细胞在胰腺癌肝转移中的保护功能研究	[79]
autoCell	高维稀疏scRNA-seq数据集的分布推断	[4]
scGCL	scRNA-seq数据估算	[82]
MCGL	scRNA-seq数据集的细胞聚类性能提高	[83]
CAME	跨物种细胞类型分配和基因模块提取	[7]
scRNA-tools	分析和处理scRNA-seq数据	[84]

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