猪传染性胃肠炎病毒感染仔猪免疫器官的组织病理学观察

doi:10.11843/j.issn.0366-6964.2023.11.041

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (11): 4872-4879.doi: 10.11843/j.issn.0366-6964.2023.11.041

猪传染性胃肠炎病毒感染仔猪免疫器官的组织病理学观察

贺叶青^1,2, 左百姣^1,2, 李庆豪^1,2, 孙娟^1,2, 金鑫^1,2, 夏璐^1,2*, 魏战勇^1,2

1. 河南农业大学动物医学院, 郑州 450046;
2. 动物病原与生物安全教育部重点实验室, 郑州 450046

收稿日期:2023-02-17 出版日期:2023-11-23 发布日期:2023-11-26
通讯作者: 夏璐,主要从事猪肠道病原体与宿主互作机制研究,E-mail:xialulwj201@163.com
作者简介:贺叶青(1998-),女,河南商丘人,硕士,主要从事猪肠道病毒致病机制研究,E-mail:heyeqing26@163.com
基金资助:
国家自然科学基金项目(32130106);"十四五"国家重点研发计划子课题(2021YFD1801103-4)

Histopathological Observation of Immune Organs of Piglets Infected with Transmissible Gastroenteritis Virus

HE Yeqing^1,2, ZUO Baijiao^1,2, LI Qinghao^1,2, SUN Juan^1,2, JIN Xin^1,2, XIA Lu^1,2*, WEI Zhanyong^1,2

1. College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China;
2. Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou 450046, China

Received:2023-02-17 Online:2023-11-23 Published:2023-11-26

摘要/Abstract

摘要： 为了探讨猪传染性胃肠炎病毒(transmissible gastroenteritis virus, TGEV)感染对仔猪免疫器官的影响,选取10只健康状况良好的5日龄仔猪,随机分为空白对照组和TGEV感染组(n=5),感染病毒5 d后剖解仔猪,采集胸腺、脾、扁桃体、腹股沟淋巴结、肠系膜淋巴结、髂下淋巴结、颈浅淋巴结和下颌淋巴结,苏木精-伊红染色观察各组织器官的病理学变化,免疫组织化学检测TGEV在免疫器官的分布。组织病理学观察发现,TGEV感染主要引起仔猪胸腺小叶肿大,免疫细胞减少;脾红髓增多,大量血细胞浸润,脾索和脾窦界限不清晰;扁桃体淋巴滤泡减少;淋巴窦中有大量血细胞浸润并伴有铁锈色。免疫组化结果显示,TGEV存在于多种免疫器官,脾中的病毒载量最高,其次是颈浅淋巴结和腹股沟淋巴结,胸腺中病毒分布最少。上述结果说明TGEV能够感染仔猪免疫系统并引起仔猪免疫器官的病理损伤,降低机体免疫功能。本研究为TGEV的免疫致病机制提供理论依据。

关键词: 猪传染性胃肠炎病毒, 仔猪, 免疫器官, 病理损伤, 分布

Abstract: In order to investigate the effects of transmissible gastroenteritis virus (TGEV) infection on the immune organs of piglets, ten healthy 5-day-old piglets were randomly divided into control group and TGEV-infected group (n=5). After 5 days of TGEV infection, the piglets were euthanized and the thymus, spleen, tonsil, inguinal lymph nodes, mesenteric lymph nodes, inferior iliac lymph nodes, superficial cervical lymph nodes and mandibular lymph nodes were collected. The pathological changes of various tissues and organs were observed by hematoxylin-eosin (HE) staining. The distribution of TGEV in various immune organs was also detected by immunohistochemistry (IHC). HE results showed TGEV infection enlarged the thymic lobules, and reduced immune cells in piglets. The red pulp was increased and filled with large numbers of blood cells. The margin between splenic cord and splenic sinus was unclear. Tonsil lymph follicles were decreased. Blood cell infiltrated in the superficial cortical area under the capsule of lymph nodes. IHC results showed that TGEV distributed in a variety of immune organs. The virus load in spleen was the highest, followed by superficial cervical lymph nodes and inguinal lymph nodes, and was least in the thymus. The above results indicate that TGEV infection affect the immune system of piglets, and cause damage to the immune system. This study provides a theoretical basis for the immune pathogenesis of TGEV.

Key words: transmissible gastroenteritis virus, piglets, immune organs, pathological injury, distribution

中图分类号:

S852.659.6

贺叶青, 左百姣, 李庆豪, 孙娟, 金鑫, 夏璐, 魏战勇. 猪传染性胃肠炎病毒感染仔猪免疫器官的组织病理学观察[J]. 畜牧兽医学报, 2023, 54(11): 4872-4879.

HE Yeqing, ZUO Baijiao, LI Qinghao, SUN Juan, JIN Xin, XIA Lu, WEI Zhanyong. Histopathological Observation of Immune Organs of Piglets Infected with Transmissible Gastroenteritis Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4872-4879.

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猪传染性胃肠炎病毒感染仔猪免疫器官的组织病理学观察

Histopathological Observation of Immune Organs of Piglets Infected with Transmissible Gastroenteritis Virus

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