低敏宠物核心料对食源性过敏小鼠的作用效果评价

doi:10.11843/j.issn.0366-6964.2022.12.029

摘要/Abstract

摘要： 本试验旨在研究低敏宠物核心料的抗过敏效果并初步探讨其可能机制。试验以5周龄雌性BALB/c小鼠为实验动物，通过OVA诱导食源性过敏小鼠模型。同时给予低敏宠物核心料，检测其对食源性过敏小鼠肠道组织结构以及过敏反应的影响，并通过检测小鼠免疫球蛋白、细胞因子、组胺水平，脾、胸腺器官指数，初步探讨其抗过敏的可能机理。结果显示：与对照组相比，食源性过敏小鼠体重增长缓慢，平均日增重极显著降低(P<0.01)，绒毛高度、隐窝深度、二者比值(V/C)均显著降低(P<0.05或P<0.01)，腹泻率极显著升高(P<0.01)，达到86%，直肠温度极显著降低(P<0.01)，过敏反应评分极显著增加(P<0.01)，血清IgE、IgG、His、IL-4水平均极显著上升(P<0.01)，IL-10水平极显著下降(P<0.01)，肥大细胞脱颗粒比率显著增加(P<0.05)，胸腺及脾指数则均极显著降低(P<0.01)；给予低敏宠物核心料的小鼠上述指标较过敏小鼠均显著改善。本试验采用的低敏宠物核心料可通过抑制肥大细胞脱颗粒，减少组织胺的释放，抑制OVA诱导的食源性小鼠过敏反应的发生，减轻过敏反应症状，同时可改善过敏小鼠肠道组织结构，促进营养物质的吸收。

关键词: 过敏, 炎性因子, 肥大细胞, 卵清蛋白, 小鼠

Abstract: The purpose of this experiment was to study the anti-allergic effect of hypoallergenic pet core material and preliminarily explore its possible mechanism. Five weeks old female BALB/c mice were used in the experimental. Ovalbumin (OVA) was administered to establish a food-borne allergy mouse model. Meanwhile, hypoallergenic pet core material was given to investigate the effect of this core material on the intestinal structure and allergic reaction of food-borne allergic mice. The anti-allergic mechanism was explored through the detection of immunoglobulin, cytokines, histamine, and immune organ index in mice. Compared with the control group, the average daily gain of food-induced allergic mice was significantly decreased (P<0.01); the villus height, crypt depth, and the ratio of which were significantly decreased (P<0.05 or P<0.01); diarrhea rate was up to 86% which was highly significantly increased (P<0.01), the rectal temperature was significantly decreased (P<0.01), and the allergic reaction score was significantly increased (P<0.01); the serum levels of IgE, IgG, His, and IL-4 were extremely significantly increased (P<0.01), the IL-10 levels were significantly decreased (P<0.01), and the number of degranulated mast cells was significantly increased (P<0.05); thymus and spleen index were significantly decreased (P<0.01); the above indexes of mice given hypoallergenic pet core material were significantly improved compared with the model mice. The hypoallergenic pet core material can inhibit the degranulation of mast cells, reduce the release of histamine, inhibit the occurrence of food induced allergic reaction in mice induced by OVA, reduce the symptoms of allergic reaction, increase the height of villi, improve the intestinal tissue structure of allergic mice and the absorption of nutrients of mice.

Key words: allergy, inflammatory factors, mast cells, ovalbumin, mice

中图分类号:

S852.55

刘佳仪, 王福正, 王承志, 杜鹏, 张明月, 陈思琪, 朱嘉欣, 孙旭, 吴高峰, 杨建成. 低敏宠物核心料对食源性过敏小鼠的作用效果评价[J]. 畜牧兽医学报, 2022, 53(12): 4439-4449.

LIU Jiayi, WANG Fuzheng, WANG Chengzhi, DU Peng, ZHANG Mingyue, CHEN Siqi, ZHU Jiaxin, SUN Xu, WU Gaofeng, YANG Jiancheng. Evaluation of the Effect of Hypoallergenic Pet Core Material on Food-borne Allergic Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4439-4449.

参考文献 38

[1]	猫咪常见过敏症有哪些？如何治疗过敏[EB/OL]. (2019-07-30). https://m.sohu.com/a/330103841_120252842/. What are common cat allergies?How to treat allergies[EB/OL]. (2019-07-30). https://m.sohu.com/a/330103841_120252842/. (in Chinese)
[2]	贺淼, 黄鑫, 陈中平, 等. 酵母水解物的消化吸收及营养作用[J]. 中国饲料, 2014(9):38-41.HE M, HUANG X, CHEN Z P, et al. The digestion and absorption of yeast hydrolyzate and its nutritional value[J]. China Feed, 2014(9):38-41. (in Chinese)
[3]	FENG J, WU M W. Effects of isoflurane on human breast cancer MDA-MB-231 cells and discussion on its relevant mechanism[J]. China Mod Doct, 2017, 55(5):8-11.
[4]	董小林, 张少君, 杨洁, 等. CD20单抗治疗侵袭性B细胞非霍奇金淋巴瘤的疗效分析[J]. 肿瘤药学, 2017, 7(3):332-335.DONG X L, ZHANG S J, YANG J, et al. Curative effect analysis of CD20 monoclonal antibody in treatment of adult patients with invasive B-cell non-Hodgkin’s lymphoma[J]. Anti-Tumor Pharmacy, 2017, 7(3):332-335. (in Chinese)
[5]	郑妍妍, 许有涛, 王洁, 等. 长链非编码RNA牛磺酸上调基因1在食管鳞状细胞癌中的表达和功能[J]. 中华实验外科杂志, 2015, 32(12):3216.ZHENG Y Y, XU Y T, WANG J, et al. Expression and function of long-chain non-coding RNA taurine upregulated gene 1 in esophageal squamous cell[J]. Chinese Journal of Experimental Surgery, 2015, 32(12):3216.(in Chinese)
[6]	WU G Y. Important roles of dietary taurine, creatine, carnosine, anserine and 4-hydroxyproline in human nutrition and health[J]. Amino Acids, 2020, 52(3):329-360.
[7]	张晓辉, 高仁元, 秦环龙. 肠道菌群与慢性病发生发展的研究进展[J]. 上海医药, 2018, 39(15):3-8, 36.ZHANG X H, GAO R Y, QIN H L. Research progress of intestinal microflora and chronic diseases[J]. Shanghai Medical & Pharmaceutical Journal, 2018, 39(15):3-8, 36. (in Chinese)
[8]	谢芳, 张海波, 幸清凤, 等. 低聚木糖对动物肠道屏障的影响及其在动物生产中应用的研究进展[J]. 中国畜牧杂志, 2020, 56(10):7-12.XIE F, ZHANG H B, XING Q F, et al. Advances in effect of xylo-oligosaccharide mediated intestinal flora and its metabolites on animal intestinal barrier[J]. Chinese Journal of Animal Science, 2020, 56(10):7-12. (in Chinese)
[9]	张红玉. 木质纤维原料醋酸催化制取低聚木糖及其纤维素酶水解[D]. 南京:南京林业大学, 2017.ZHANG H Y. Acetic acid prehydrolysis, enzyme hydrolysis of lignocellulose for xylooligosaccharides and fermentable sugars production[D]. Nanjing:Nanjing Forestry University, 2017. (in Chinese)
[10]	CRAIG J M. Food intolerance in dogs and cats[J]. J Small Anim Pract, 2019, 60(2):77-85.
[11]	NURMATOV U, DHAMI S, ARASI S, et al. Allergen immunotherapy for IgE-mediated food allergy:a systematic review and meta-analysis[J]. Allergy, 2017, 72(8):1133-1147.
[12]	YU W, FREELAND D M H, NADEAU K C. Food allergy:immune mechanisms, diagnosis and immunotherapy[J]. Nat Rev Immunol, 2016, 16(12):751-765.
[13]	马玉. 橄榄油对OVA诱导小鼠食物过敏的干预作用及调控机制研究[D]. 厦门:集美大学, 2021.MA Y. The intervention effect and regulation mechanism of olive oil on OVA-induced food allergy in mice[D]. Xiamen:Jimei University, 2021. (in Chinese)
[14]	熊志乐. 表面表达霍乱毒素B与卵清白蛋白枯草芽孢预防OVA所致食物过敏的研究[D]. 广州:广州医科大学, 2021.XIONG Z L. Recombinant bacillus subtilis spores expressing cholera toxin B and ovalbumin protein prevent against OVA-induced food allergy in mice[D]. Guangzhou:Guangzhou Medical University, 2021. (in Chinese)
[15]	YE P, YANG X L, CHEN X, et al. Hyperoside attenuates OVA-induced allergic airway inflammation by activating Nrf2[J]. Int Immunopharmacol, 2017, 44:168-173.
[16]	余熠, 苏雯, 张清清, 等. 肠道微生态在食物过敏发生及防治中的作用[J]. 临床儿科杂志, 2021, 39(8):636-640.YU Y, SU W, ZHANG Q Q, et al. Role of intestinal microecology in the occurrence and prevention of food allergies[J]. Journal of Clinical Pediatrics, 2021, 39(8):636-640. (in Chinese)
[17]	LIANG L, HUR J, KANG J Y, et al. Effect of the anti-IL-17 antibody on allergic inflammation in an obesity-related asthma model[J]. Korean J Intern Med, 2018, 33(6):1210-1223.
[18]	YAN X M, YAN J B, XIANG Q W, et al. Fructooligosaccharides protect against OVA-induced food allergy in mice by regulating the Th17/Treg cell balance using tryptophan metabolites[J]. Food Funct, 2021, 12(7):3191-3205.
[19]	谢红兵, 邹云, 刘丽莉, 等. 植物多糖对断奶仔猪小肠黏膜形态及肠黏膜屏障功能的影响[J]. 中国兽医学报, 2019, 39(1):150-157, 187.XIE H B, ZOU Y, LIU L L, et al. Effects of botanic polysaccharides on intestinal mucosal morphology and intestinal barrier function in weaned piglets[J]. Chinese Journal of Veterinary Science, 2019, 39(1):150-157, 187. (in Chinese)
[20]	张佳琦, 熊玮, 黎智华, 等. 芦丁对断奶仔鼠生长、肠道抗氧化及脂代谢的影响[J]. 南京农业大学学报, 2022, 45(2):368-376.ZHANG J Q, XIONG W, LI Z H, et al. Effects of rutin on growth, intestinal antioxidant capacity and lipid metabolism in weaned mice[J]. Journal of Nanjing Agricultural University, 2022, 45(2):368-376. (in Chinese)
[21]	WEIDMANN E, SAMADI N, KLEMS M, et al. Mouse chow composition influences immune responses and food allergy development in a mouse model[J]. Nutrients, 2018, 10(11):1775.
[22]	LI N, YU Y, CHEN X H, et al. Bifidobacterium breve M-16V alters the gut microbiota to alleviate OVA-induced food allergy through IL-33/ST2 signal pathway[J]. J Cell Physiol, 2020, 235(12):9464-9473.
[23]	TRINCHESE G, PAPARO L, AITORO R, et al. Hepatic mitochondrial dysfunction and immune response in a murine model of peanut allergy[J]. Nutrients, 2018, 10(6):744.
[24]	DIESNER S C, BERGMAYR C, PFITZNER B, et al. A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model[J]. Clin Immunol, 2016, 173:10-18.
[25]	REN J J, ZHAO Y, HUANG S, et al. Immunomodulatory effect of Bifidobacterium breve on experimental allergic rhinitis in BALB/c mice[J]. Exp Ther Med, 2018, 16(5):3996-4004.
[26]	喻波明. 两种海藻多糖的免疫调节作用研究[D]. 深圳:深圳大学, 2019.YU B M. Study on the immunomodulatory effects of two seaweed polysaccharides[D]. Shenzhen:Shenzhen University, 2019. (in Chinese)
[27]	MARCO-MARTÍN G, LA ROTTA HERNÁNDEZ A, VÁZQUEZ DE LA TORRE M, et al. Differences in the anaphylactic response between C3H/HeOuJ and BALB/c mice[J]. Int Arch Allergy Immunol, 2017, 173(4):204-212.
[28]	GUPTA R K, SHARMA A, VERMA A, et al. Inherent allergic potential of α-dioxygenase fragment:a pathogenesis related protein[J]. Immunobiology, 2019, 224(2):207-219.
[29]	BARLETTA B, ROSSI G, SCHIAVI E, et al. Probiotic VSL#3-induced TGF-β ameliorates food allergy inflammation in a mouse model of peanut sensitization through the induction of regulatory T cells in the gut mucosa[J]. Mol Nutr Food Res, 2013, 57(12):2233-2244.
[30]	FU Y W, WANG Y D, GAO H, et al. Associations among dietary omega-3 polyunsaturated fatty acids, the gut microbiota, and intestinal immunity[J]. Mediat Inflamm, 2021, 2021:8879227.
[31]	GUTIRREZ S, SVAHN S L, JOHANSSON M E. Effects of omega-3 fatty acids on immune cells[J]. Int J Mol Sci, 2019, 20(20):5028.
[32]	COSTANTINI L, MOLINARI R, FARINON B, et al. Impact of omega-3 fatty acids on the gut microbiota[J]. Int J Mol Sci, 2017, 18(12):2645.
[33]	陆锦锐. 湿疹清颗粒对慢性湿疹模型大鼠作用及机制初探[D]. 昆明:云南中医药大学, 2020.LU J R. Effect of Shizhenqing granule on chronic eczema model rats and preliminary mechanism[D]. Kunming:Yunnan University of Traditional Chinese Medicine, 2020. (in Chinese)
[34]	刘玮, 韩海霞, 曹顶国, 等. α-多肽菌素对科宝500肉鸡生产性能、免疫指标、血清生化指标和抗氧化性能的影响[J]. 中国家禽, 2018, 40(4):29-35.LIU W, HAN H X, CAO D G, et al. Effect of α-polypeptin on growth performance, immune indexes, serum biochemical indexes and antioxidant properties of cobb 500 broilers[J]. China Poultry, 2018, 40(4):29-35. (in Chinese)
[35]	ASLAN KARAKELLE N, DINCER S, YAR SAǦLAM A S. The effect of intracerebroventricular amyloid beta 1-42 application on cognitive functions in aged rats supplemented with taurine and the change of peroxisomal proteins in this process[J]. Brain Res Bull, 2021, 172:89-97.
[36]	ABDEL-DAIM M M, DESSOUKI A A, ABDEL-RAHMAN H G, et al. Hepatorenal protective effects of taurine and N-acetylcysteine against fipronil-induced injuries:the antioxidant status and apoptotic markers expression in rats[J]. Sci Total Environ, 2019, 650(Pt 2):2063-2073.
[37]	ZHANG M, YANG H Y, YANG E W, et al. Berberine decreases intestinal GLUT2 translocation and reduces intestinal glucose absorption in mice[J]. Int J Mol Sci, 2021, 23(1):327.
[38]	SCHAFFER S W, SHIMADA-TAKAURA K, JONG C J, et al. Impaired energy metabolism of the taurine-deficient heart[J]. Amino Acids, 2016, 48(2):549-558.