牛凸隆病毒HE基因分子特征分析

doi:10.11843/j.issn.0366-6964.2021.07.029

摘要/Abstract

摘要： 本研究旨在分析我国牛凸隆病毒（bovine torovirus，BToV）的HE基因分子特征。采用RT-PCR方法检测辽宁、河南、四川的牛腹泻样本中BToV，阳性样本扩增完整HE基因。结果显示：从94份腹泻粪便中检出10份BToV阳性，平均阳性率约为10.64%。从10份阳性样本中扩增得到15条HE基因，其中，9条为基因Ⅱ型，6条为基因Ⅲ型；10份阳性样本中有5份存在Ⅲ型和Ⅱ型毒株的混合感染，1份单独感染Ⅲ型毒株，4份单独感染Ⅱ型毒株。与GenBank中其他Ⅲ型HE基因相比，本研究获得的6条Ⅲ型HE基因具有4个相同的氨基酸突变，其中2个位于凝集素结构域，可能会影响受体结合功能。重组分析表明，BToV Ⅲ型毒株可能是Ⅱ型毒株与Ⅰ型毒株重组而来。本研究首次证实了基因Ⅲ型BToV在我国牛群中存在，报道了Ⅲ型与Ⅱ型毒株混合感染的情况，为国内牛腹泻病的防控和BToV的遗传进化研究提供了参考。

关键词: 牛凸隆病毒, HE基因, 基因Ⅲ型, 重组

Abstract: The purpose of this study was to characterize the molecular characteristics of the HE genes of bovine torovirus (BToV). An RT-PCR assay was used to detect BToV from 94 diarrheic fecal samples collected in Liaoning, Henan, and Sichuan provinces and full-length hemagglutinin-esterase (HE) genes were further amplified from the positive samples. Approximate 10.64% (10/94) samples were detected as BToV positive, and 15 full-length HE genes were sequenced from 10 positive samples, including 9 genotype Ⅱ strains and 6 genotype Ⅲ strains. Among the 10 positive samples, 5 samples were co-infected with genotype Ⅱ and Ⅲ strains, 1 sample was infected with genotype Ⅲ only and 4 samples were infected with genotype Ⅱ strains only. Compared with other available genotype Ⅲ BToV HE genes in the GenBank database, 4 unique amino acid variations were found in the HE gene, including 2 variations in the lectin domain which may affect HE receptor binding. Recombination analysis showed that BToV genotype Ⅲ strains may be derived from the recombination of genotype Ⅱand genotype Ⅰstrains in the HE gene. This study is the first confirmation of genotype Ⅲ BToV in China and the co-infection of genotype Ⅱ and Ⅲ strains. These findings contributed to the diagnosis and control of bovine diarrhea in China and enhance the current understanding of the genetic evolution of BToV.

Key words: bovine torovirus, HE gene, genotype Ⅲ, recombination

中图分类号:

S852.659.6

赵龙, 汤承, 岳华. 牛凸隆病毒HE基因分子特征分析[J]. 畜牧兽医学报, 2021, 52(7): 2065-2072.

ZHAO Long, TANG Cheng, YUE Hua. Molecular Characteristics Analysis of HE Genes of Bovine Torovirus[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2065-2072.

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