肺离子细胞相关蛋白ATP6V0D2在不同年龄牦牛肺内分布和表达

doi:10.11843/j.issn.0366-6964.2021.06.032

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (6): 1765-1768.doi: 10.11843/j.issn.0366-6964.2021.06.032

• 研究简报 • 上一篇

肺离子细胞相关蛋白ATP6V0D2在不同年龄牦牛肺内分布和表达

魏雅婷, 崔燕, 张倩, 何俊峰*

甘肃农业大学动物医学院甘肃省牛羊胚胎工程技术研究中心, 兰州 730070

收稿日期:2020-09-22 出版日期:2021-06-23 发布日期:2021-06-22
通讯作者: 何俊峰,主要从事动物组织学与胚胎学研究,E-mail:hejf@gsau.edu.cn
作者简介:魏雅婷(1994-),女,甘肃榆中人,硕士,主要从事动物组织学与胚胎学研究,E-mail:1432162405@qq.com
基金资助:
国家自然科学基金（31960687）；甘肃农业大学青年导师基金资助项目（GAU-QNDS-201708）

Distribution and Expression of Pulmonary Ionocyte-related Factor ATP6V0D2 in the Lungs of Yaks at Different Ages

WEI Yating, CUI Yan, ZHANG Qian, HE Junfeng*

Research Center of Bovine and Sheep Embryo Engineering Technology of Gansu Province, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received:2020-09-22 Online:2021-06-23 Published:2021-06-22

摘要/Abstract

摘要： 本研究旨在揭示肺离子细胞相关蛋白ATP6V0D2在不同年龄组牦牛肺中的分布和表达特点，探索ATP6V0D2在牦牛肺适应高原低氧环境中的结构形成和可能调控作用。利用免疫组织化学，qRT-PCR和Western blot对初生、幼年、成年和老年4个年龄组牦牛肺中ATP6V0D2的准确分布位置及相对表达量进行研究。结果显示，ATP6V0D2主要分布在肺内支气管及其分支的管壁上皮黏膜层的纤毛细胞和克拉拉细胞中；在成年组和老年组呈强阳性表达，ATP6V0D2在不同年龄组牦牛肺中均有不同程度的表达，以初生组表达量最高，与其他3组相比较，差异性显著（P<0.05）。在蛋白水平上，ATP6V0D2在成年组中的表达量显著高于其他3组（P<0.05）；初生组、幼年组、老年组两两相比较，差异性均显著（P<0.05）。ATP6V0D2在不同年龄牦牛肺细支气管上皮细胞中均有表达，但不同年龄组间的表达存在显著差异（P<0.05）。肺离子细胞相关蛋白ATP6V0D2表达量的不同与牦牛肺对低氧环境的适应性过程有关，纤毛细胞和克拉拉细胞与肺离子细胞的功能具有相关性，在牦牛肺适应高原低氧环境、防止气道损伤或修复过程方面发挥重要作用。

关键词: 牦牛, 肺, 肺离子细胞, ATP6V0D2

Abstract: To reveal the distribution and expression characteristics of the pulmonary ionocyte-related factor ATP6V0D2 in the lungs of yaks of different ages and to explore the structural formation and possible regulation of ATP6V0D2 in the yak lung for adaptation to high-altitude hypoxia. The localization and expression of ATP6V0D2 in the lungs of newborn, juvenile, adult, and elderly yaks were studied using immunohistochemistry, quantitative reverse transcription PCR (qRT-PCR), and Western blot. The results of immunohistochemistry showed that ATP6V0D2 was mainly localized in the ciliated cells and club cells of the epithelial mucosal layer of the bronchus and its branches in the lungs, and it was strongly expressed in the adult group and elderly. Expression of ATP6V0D2 in the yak lungs varied according to age. The ATP6V0D2 expression was the highest in the newborn group and was significantly different from the other three groups (P<0.05). The ATP6V0D2 protein expression of the adult group was significantly higher than the other three groups (P<0.05), and the pairwise comparisons between the newborn, juvenile, and elderly groups showed significant differences (P<0.05). ATP6V0D2 was expressed in bronchiolar epithelial cells in the lungs of yaks at different ages, and the expression levels significantly varied among different age groups(P<0.05). This study showed that ciliated cells and club cells were related to the pulmonary ionocytes in yaks. The differences in pulmonary ionocyte-related factors ATP6V0D2 was related to adaptations of yak lungs to high altitude hypoxia, through prevention of airway damage.

Key words: yak, lung, pulmonary ionocyte, ATP6V0D2

中图分类号:

S823.85

魏雅婷, 崔燕, 张倩, 何俊峰. 肺离子细胞相关蛋白ATP6V0D2在不同年龄牦牛肺内分布和表达[J]. 畜牧兽医学报, 2021, 52(6): 1765-1768.

WEI Yating, CUI Yan, ZHANG Qian, HE Junfeng. Distribution and Expression of Pulmonary Ionocyte-related Factor ATP6V0D2 in the Lungs of Yaks at Different Ages[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1765-1768.

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肺离子细胞相关蛋白ATP6V0D2在不同年龄牦牛肺内分布和表达

Distribution and Expression of Pulmonary Ionocyte-related Factor ATP6V0D2 in the Lungs of Yaks at Different Ages

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