犬正常发情期与患子宫蓄脓的子宫和卵巢组织结构及乳铁蛋白表达的对比

doi:10.11843/j.issn.0366-6964.2021.04.026

摘要/Abstract

摘要： 本研究旨在探明犬子宫和卵巢在正常发情期与患子宫蓄脓时，其组织结构及乳铁蛋白（lactoferrin，LF）表达的变化特征。应用Masson’s、VVG、PAS组织化学染色方法观察乏情期、发情期、患子宫蓄脓时犬子宫及卵巢的组织结构特点，用免疫组织化学SP法观察LF的分布特征。结果显示，正常发情期犬：乏情期、发情期子宫内膜肌层厚度比分别为0.762 0、0.924 3；乏情期子宫固有层中胶原纤维含量大于发情期，发情期卵巢中胶原纤维含量大于乏情期；子宫血管层及卵巢血管内弹性膜清晰完整；子宫腺在乏情期时浅层管腔小，深层较大、腺管上皮为单层柱状上皮、上皮细胞及管腔内PAS阳性反应较强，发情期子宫腺管腔变大、腺管上皮为单层立方上皮、上皮细胞及管腔内有PAS阳性反应；子宫黏膜上皮在乏情期和发情期均为单层柱状上皮，但胞核位置不同，乏情期胞核位于中央，发情期胞核位于顶部。患子宫蓄脓犬：子宫内膜肌层厚度比为1.615 0；子宫固有层和卵巢中胶原纤维含量少于正常发情期；子宫腺管腔大，且形状不规则，管腔内有炎性细胞浸润，腺管上皮为单层立方上皮，有淋巴细胞位于基膜，上皮细胞及管腔内PAS阳性反应较弱；子宫血管层及卵巢血管内弹性膜较正常发情期变薄，且有断裂现象；子宫黏膜上皮为单层柱状上皮，胞核位于基底。LF在乏情期子宫腺上皮和卵巢中的表达水平高于发情期，而在子宫黏膜上皮中发情期的表达水平高于乏情期。患子宫蓄脓时犬子宫和卵巢中LF的表达水平均较低。综上表明，犬正常发情期与患子宫蓄脓时的子宫和卵巢组织结构特点显著不同，LF的表达水平也存在差异。

关键词: 正常发情期, 子宫蓄脓, 犬子宫和卵巢, 组织结构, 乳铁蛋白表达

Abstract: The purpose of this study was to investigate the changes in histological structure and the expression of lactoferrin (LF) in the uterus and ovaries of dogs during the normal estrus and with pyometra. Masson’s, VVG and PAS histochemical staining were used to observe the histological characteristics of uteruses and ovaries in anestrus, estrus, and pyometra. The immunohistochemical SP method was used to observe the distribution of LF. The results showed that the endometrium/myometrium ratios in the period of anestrus and estrus were 0.762 0 and 0.924 3, respectively. The content of collagen fibers in the uterine lamina propria in anestrus was higher than that in estrus, and the collagen fibers content of ovary in estrus was higher than that in anestrus. The internal elastic membranes of uterine vascular layer and ovarian blood vessels were clear and intact in anestrus and estrus. During the period of anestrus, the superficial lumen was small and the deep lumen was large, the epithelium of glandular duct was consisted of the single layered columnar epitheliums, and the PAS positive reaction was strong in epithelial cells and glandular cavity. During the period of estrus, the glandular lumen became larger, the glandular epithelium was the single layered cuboidal epithelium, and PAS positive reaction was found in the epithelial cells and glandular lumen. The endometrial epithelium was monolayer columnar epithelium in both estrus and anestrus periods, but the position of the nucleus was different. The endometrial epithelium nucleus of anestrus period was located in the center of cell, the endometrial epithelium nucleus of estrus period was at the top of cell. When the dogs have pyometra, the endometrium/myometrium ratio was 1.615 0, the content of collagen fibers in the lamina propria of uteruses and ovaries was less than that during normal estrus, the glandular lumen was large and irregular in shape, the inflammatory cells infiltrated in the lumen, the glandular epithelium was the single layered cuboidal epithelium, the lymphocytes were located in the basement membrane, and PAS positive reaction was weak in epithelial cells and lumen; the elastic membrane in uterine vascular layer and ovarian blood vessels became thinner and fractured compared during normal estrus; the endometrial epithelium was the single monolayer columnar epithelium, and the nucleus was located at basal plasma membrane. The expression level of LF in uterine glandular epithelium and ovaries in anestrus stage was higher than that in estrus period, and the expression level of LF in endometrial epithelium in estrus was higher than that in anestrus. The expression of LF in uteruses and ovaries of dogs with pyometra was lower. The results suggested that the histological characteristics of uteruses and ovaries of dogs in normal estrus and pyometra were significantly different, and the expression level of LF was also different.

Key words: normal estrous stages, pyometra, dog uterus and ovary, histological structure, lactoferrin

中图分类号:

S857.23

刘雨珂, 徐倩倩, 孙佩芳, 陈美伊, 刘铭峰, 段德勇. 犬正常发情期与患子宫蓄脓的子宫和卵巢组织结构及乳铁蛋白表达的对比[J]. 畜牧兽医学报, 2021, 52(4): 1103-1114.

LIU Yuke, XU Qianqian, SUN Peifang, CHEN Meiyi, LIU Mingfeng, DUAN Deyong. Comparison of Histological Structure and Lactoferrin Expression in Uterine and Ovarian of Dogs during Normal Estrus and with Pyometra[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1103-1114.

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