牛瘤胃上皮细胞中挥发性脂肪酸吸收相关蛋白在白细胞介素10诱导下的差异表达研究

doi:10.11843/j.issn.0366-6964.2021.03.015

摘要/Abstract

摘要： 本研究旨在探讨炎症因子（白细胞介素10， IL-10）对牛瘤胃上皮细胞中挥发性脂肪酸（VFA）吸收相关蛋白基因表达的影响。试验采用随机区组设计，在瘤胃上皮细胞体外培养条件下，研究50 ng·mL^-1浓度的IL-10对牛瘤胃上皮细胞中核因子（NF-κB）、假定阴离子转运蛋白1（PAT1）、阴离子交换蛋白2（AE2）、单羧酸转运蛋白1（MCT1）、单羧酸转运蛋白4（MCT4）、钠氢离子交换蛋白1（NHE1）基因表达的影响。结果表明，在中性条件下，IL-10添加显著下调了NF-κB、PAT1、MCT1、NHE1基因的表达（P<0.05），AE2基因的表达显著性上调（P<0.05），而对MCT4基因的表达没有显著影响。在酸性条件下，IL-10添加组的NF-κB基因表达被显著性抑制（P<0.05），而PAT1、MCT1、MCT4基因表达显著上调（P<0.05）。pH5.5组与pH7.2组比较，pH5.5组NF-κB基因表达显著性上调（P<0.05）。结果提示，在炎症条件下，白细胞介素10作为一种抗炎因子，可以缓解瘤胃上皮细胞炎症反应从而促进VFA吸收相关蛋白基因的表达。

关键词: IL-10, 瘤胃上皮细胞, 载体蛋白, 挥发性脂肪酸

Abstract: This study aimed to investigate the effect of inflammatory factor (interleukin-10, IL-10) on the expression of genes related to volatile fatty acid (VFA) absorption in bovine rumen epithelial cells. The experiment adopted a randomized block design. Under the conditions of in vitro culture of rumen epithelial cells, the effect of 50 ng·mL^-1 IL-10 on genes expression of nuclear factor (NF-κB), putative anion transporter 1 (PAT1), anion exchanger 2(AE2), monocarboxylate transporter 1(MCT1), monocarboxylate transporter 4 (MCT4), Na^-/H⁺exchanger 1 (NHE1) in bovine rumen epithelial cells was detected. The results showed that, under neutral conditions, IL-10 addition significantly down-regulated the expression of NF-κB, PAT1, MCT1, NHE1 genes (P<0.05), and significantly up-regulated the expression of AE2 gene (P<0.05), while had no significant effect on the gene expression of MCT4. Under acidic conditions, the expression of NF-κB gene in the IL-10 addition group was significantly inhibited (P<0.05), while the expression of PAT1, MCT1, and MCT4 genes were significantly promoted (P<0.05). Compared with the condition of pH7.2 group, the NF-κB gene expression in the pH5.5 group was significantly increased (P<0.05). The results suggest that, under inflammatory conditions, interleukin-10 as an anti-inflammatory factor can alleviate the inflammatory response of rumen epithelial cells and promote the genes expression of VFA absorption-related protein.

Key words: IL-10, rumen epithelial cells, carrier protein, volatile fatty acids

中图分类号:

S823.2

侯红雁, 王忠豪, 霍文婕, 刘强, 张静, 陈雷, 裴彩霞, 郭刚. 牛瘤胃上皮细胞中挥发性脂肪酸吸收相关蛋白在白细胞介素10诱导下的差异表达研究[J]. 畜牧兽医学报, 2021, 52(3): 714-722.

HOU Hongyan, WANG Zhonghao, HUO Wenjie, LIU Qiang, ZHANG Jing, CHEN Lei, PEI Caixia, GUO Gang. Differential Expression of Volatile Fatty Acid Absorption Related Proteins in Bovine Rumen Epithelial Cells Induced by Interleukin 10[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 714-722.

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