板芩桔甘合剂对传染性支气管炎病毒人工感染雏鸡的临床疗效分析

doi:10.11843/j.issn.0366-6964.2020.02.020

摘要/Abstract

摘要： 旨在探讨板芩桔甘合剂（BQJGHJ）对传染性支气管炎病毒（IBV）人工感染雏鸡的临床疗效。选取120只雏鸡随机分为空白组、模型组（感染病毒）、阳性药物组（感染病毒+甘胆口服液）和BQJGHJ高、中、低剂量组（感染病毒+BQJGHJ），每组20只。除空白组外，其他组感染IBV造模，4 d后，各组分别饮水给药，连用5 d。每天观察各组雏鸡临床症状，记录临床症状记分，统计有效率、治愈率；在造模后，给药第3、5天，及停药后第2天分别自翅下静脉采集血液，分离血清，ELISA法检测感染雏鸡血清中IFN-γ、IL-6、IL-10含量变化。结果显示：BQJGHJ高、中、低剂量组的治愈率分别为75.0%、80.0%、65.0%，有效率分别为90.0%、95.0%、80.0%。模型组雏鸡血清中IL-10含量降低，IL-6含量升高，与空白组比较，差异显著（P<0.05或P<0.01）；在给药第3、5天时，BQJGHJ高、中、低剂量组、阳性药物组雏鸡血清中IFN-γ（第5天时，高剂量组差异极显著，P<0.01，其他时间、其他组差异均不显著）、IL-10含量（第5天时差异极显著，P<0.01）升高，雏鸡血清中IL-6含量降低，与模型组比较，均差异显著（P<0.05）。在停药后2 d，BQJGHJ高、中、低剂量组、阳性药物组雏鸡血清中IFN-γ、IL-10含量升高，雏鸡血清中IL-6含量降低，与模型组比较，均差异显著（P<0.05或P<0.01）；BQJGHJ高、中、低剂量组雏鸡血清中IFN-γ、IL-6、IL-10含量，与阳性药物组相比，无显著性差异（P>0.05）。BQJGHJ可提高传染性支气管炎雏鸡血清中IFN-r、IL-10的含量，降低传染性支气管炎雏鸡血清中IL-6的含量，发挥治疗鸡传染性支气管炎的作用。

关键词: 板芩桔甘合剂, IBV-M41, IFN-γ, IL-6, IL-10

Abstract: This study was conducted to investigate the clinical efficacy of BQJGHJ, a traditional Chinese medicine compound pharmaceutics, in the treatment of chickens artificially infected with Infectious bronchitis virus (IBV). One hundred and twenty chicks were divided into blank group, model group (IBV infection), positive drug group (IBV infection & Gan Dan oral liquid), and BQJGHJ high, middle and low dose groups (IBV infection & BQJGHJ), 20 chicks in each group. Except for the blank group,the other groups were modeled by infecting IBV-M41 strain, 4 days after modeling, the medicines were administrated through drinking water for 5 days. The clinical symptoms of each group were observed every day, the scores of clinical symptoms were recorded, the effective rate was counted, and the cure rate was used as the evaluation index. On the 3rd, 5th and 7th day post the first administration, the blood samples were collected from the wing vein and the serum were separated. The contents of IFN-γ, IL-6 and IL-10 in chicks serum were detected by ELISA. The results indicated that the cure rates of BQJGHJ high, middle and low dose groups were 75.0%, 80.0% and 65.0%, and the effective rates were 90.0%, 95.0% and 80.0%, respectively. The content of IL-10 in serum of chicks in model group decreased and the content of IL-6 increased, which was significantly different from that in blank group (P<0.05 or P<0.01). On day 3 and 5 of administration, the contents of IFN-γ (P<0.01 at day 5 in high dose group,P>0.05 for others) and IL-10(P<0.01 at day 5 in eath BQJGHJ gruop and positive drug group) in serum of chicks in BQJGHJ high, middle and low dose groups and positive drug groups increased, while the contents of IL-6 in serum of chicks decreased, which were significantly different from those in model group (P<0.05). On the 2nd day after withdrawal, the contents of IFN-γ and IL-10 in serum of chicks in BQJGHJ high, middle and low dose groups and positive drug groups increased, while the contents of IL-6 in serum decreased, which were significantly different from those in model group (P<0.05 or P<0.01); There was no significant difference in the contents of IFN-γ and IL-6, IL-10 in serum of chicks between each BQJGHJ group and the positive drug group (P>0.05). BQJGHJ can increase the content of IFN-γ,IL-10 in serum of chicks with infectious bronchitis, reduce the content of IL-6 in serum of chicks with infectious bronchitis, so as to achieve the effect of treating infectious bronchitis in chicks.

Key words: BQJGHJ, IBV-M41, IFN-γ, IL-6, IL-10

中图分类号:

S853.9

林春发, 罗艺晨, 王忠清, 冯静波, 刘娟. 板芩桔甘合剂对传染性支气管炎病毒人工感染雏鸡的临床疗效分析[J]. 畜牧兽医学报, 2020, 51(2): 382-391.

LIN Chunfa, LUO Yichen, WANG Zhongqing, FENG Jingbo, LIU Juan. The Clinical Efficacy of BQJGHJ (a Traditional Chinese Medicine Compound Pharmaceutics) in the Treatment of Chickens Artificially Infected with Infectious Bronchitis Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 382-391.

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