妊娠早期饲粮添加不同水平N-氨甲酰谷氨酸改善母羊产羔性能的研究

doi:10.11843/j.issn.0366-6964.2021.013

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (1): 126-134.doi: 10.11843/j.issn.0366-6964.2021.013

妊娠早期饲粮添加不同水平N-氨甲酰谷氨酸改善母羊产羔性能的研究

蔡元¹, 田见晖², 王栋³, 罗玉柱^4*, 翁士乔⁵, 李春阳¹

1. 西北民族大学生命科学与工程学院, 兰州 730030;
2. 中国农业大学动物科学技术学院, 北京 100193;
3. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
4. 甘肃农业大学动物科学技术学院, 兰州 730070;
5. 宁波三生生物科技有限公司, 宁波 315100

收稿日期:2020-03-11 出版日期:2021-01-23 发布日期:2021-01-19
通讯作者: 罗玉柱,主要从事动物遗传育种与繁殖研究,E-mail:luoyz@gsau.edu.cn
作者简介:蔡元(1976-),男,甘肃武威人,副教授,硕士生,主要从事动物遗传育种与繁殖研究,E-mail:413432905@qq.com
基金资助:
“十三五”国家重点研发计划课题（2017YFD0501904）；中央高校基本科研业务费专项资金项目（31920190043）；甘肃农业大学课题（GSAU-ZL-2015-031）；中央高校基本科研业务费专项资金项目（31920190208-02）；兰州市科技计划项目（2016-3-91）

Effects of Dietary N-carbamylglutamate Supplementation on Improving Lambing Performance of Ewes during Early Pregnancy

CAI Yuan¹, TIAN Jianhui², WANG Dong³, LUO Yuzhu^4*, WENG Shiqiao⁵, LI Chunyang¹

1. Life Science and Engineering College of Northwest Minzu University, Lanzhou 730030, China;
2. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
3. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
4. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
5. Ningbo Sansheng Biotechnology Co., Ltd., Ningbo 315100, China

Received:2020-03-11 Online:2021-01-23 Published:2021-01-19

摘要/Abstract

摘要： 旨在研究妊娠早期饲喂不同水平N-氨甲酰谷氨酸（N-carbamylglutamate，NCG）对母羊产羔性能及相关血液指标的影响。选用2~3胎次湖羊160只，随机分为4组，每组40只母羊，自配种当天（记为妊娠第0天）开始，分别饲喂基础饲粮（对照组）、基础饲粮+0.05% NCG、基础饲粮+0.08% NCG、基础饲粮+0.11% NCG，40 d后均饲喂基础饲粮。测定母羊产羔性能和妊娠第0、20、40天母羊血浆中游离氨基酸、总一氧化氮合酶（TNOS）、诱导型一氧化氮合酶（iNOS）、一氧化氮（NO）、生长激素、雌二醇、孕酮、尿素和氨浓度。结果显示，0.08%和0.11% NCG组产羔性能显著提高，两组的窝产羔数和窝产活羔数较对照组显著增加（P<0.05），但活羔初生个体重显著降低（P<0.05）；各NCG组妊娠第40天NO浓度显著升高（P<0.05），0.11% NCG组妊娠第40天iNOS浓度和妊娠第20天孕酮浓度较对照组显著升高（P<0.05）；0.08%和0.11% NCG组妊娠第20天氨浓度显著低于对照组和0.05% NCG组（P<0.05），0.11% NCG组妊娠第40天氨浓度显著低于对照组（P<0.05）。综上，妊娠早期母羊饲粮添加NCG可提高母羊体循环iNOS、NO和孕酮浓度，改善胎盘营养与妊娠维持状态；降低血浆氨浓度，提高胎儿发育环境安全性，改善母羊产羔性能，提高母羊窝产羔数和窝产活羔数。

关键词: N-氨甲酰谷氨酸, 经产母羊, 产羔性能, 血液指标

Abstract: The aim of the present study is to investigate the effects of dietary N-carbamylglutamate (NCG) supplementation on lambing performance and blood indexes of ewes during early pregnancy. A total of 160 second- and third-parity Hu sheep were randomly assigned into 4 groups which fed with basal diet(control group), basal diet supplemented with 0.05%, 0.08% and 0.11%NCG, respectively. Sheep were fed with experimental diets for 40 days from the day of mating. After that, all sheep were fed with basal diet. The parameters of lambing performance were recorded immediately after farrowing. The plasma amino acids, total nitric oxide synthase (TNOS), inducible nitric oxide synthase (iNOS), nitric oxide (NO), growth hormone, estradiol, progesterone, urea and ammonia were detected on the day 0, 20 and 40 of pregnancy. The results showed that the dietary supplementation of 0.08% and 0.11%NCG significantly increased the number of total lambs and total live lambs per litter compared with the control group(P<0.05), but the birth weight of lamb born alive decreased significantly (P<0.05). On day 40 of pregnancy, the plasma concentration of NO in NCG-supplemented groups were significantly higher than that of control group(P<0.05). In addition,compared to control group, 0.11% NCG-supplemented group had higher plasma concentration of iNOS on day 40 of pregnancy (P<0.05), as well as higher plasma concentration of progesterone on day 20 of pregnancy (P<0.05). Moreover, both 0.08% and 0.11% NCG -supplemented groups had lower level of plasma ammonia than those in control group and 0.05% NCG-supplemented group on day 20 of pregnancy (P<0.05), and on day 40 of pregnancy the plasma ammonia in 0.11% NCG-supplemented group was significantly lower than that of control group (P<0.05). The results suggested that dietary NCG supplementation during the early stage of pregnancy increased plasma concentration of iNOS, NO and progesterone, and thus improved placental nutrition and pregnancy maintenance. The reduced plasma concentration of ammonia by NCG supplementation, may improve the lambing performance by optimizing the uterine environment and enhancing fetal developmental potential.

Key words: N-carbamylglutamate, multiparous ewes, lambing performance, blood indexes

中图分类号:

S814
S816.79

蔡元, 田见晖, 王栋, 罗玉柱, 翁士乔, 李春阳. 妊娠早期饲粮添加不同水平N-氨甲酰谷氨酸改善母羊产羔性能的研究[J]. 畜牧兽医学报, 2021, 52(1): 126-134.

CAI Yuan, TIAN Jianhui, WANG Dong, LUO Yuzhu, WENG Shiqiao, LI Chunyang. Effects of Dietary N-carbamylglutamate Supplementation on Improving Lambing Performance of Ewes during Early Pregnancy[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 126-134.

参考文献 37

[1]	BAZER F W,FIRST N L.Pregnancy and parturition[J].J Anim Sci,1983,57 Suppl 2:425-460.
[2]	ZHANG H,SUN L W,WANG Z Y,et al.N-carbamylglutamate and L-arginine improved maternal and placental development in underfed ewes[J].Reproduction,2016,151(6):623-635.
[3]	ROSSELLI M,KELLER R J,DUBEY R K.Role of nitric oxide in the biology,physiology and path-ophysiology of reproduction[J].Hum Reprod Update,1998,4(1):3-24.
[4]	KRAUSE B J,HANSON M A,CASANELLO P.Role of nitric oxide in placental vascular development and function[J].Placenta,2011,32(11):797-805.
[5]	KAUFMANN P,BLACK S,HUPPERTZ B.Endovascular trophoblast invasion:implications for the pathogenesis of intrauterine growth retardation and preeclampsia[J].Biol Reprod,2003,69(1):1-7.
[6]	AYLING L J,WHITLEY G S J,APLIN J D,et al.Dimethylarginine dimethylaminohydrolase (DDAH) regulates trophoblast invasion and motility through effects on nitric oxide[J].Hum Reprod,2006,21(10):2530-2537.
[7]	HARRIS L K,MCCORMICK J,CARTWRIGHT J E,et al.S-nitrosylation of proteins at the leading edge of migrating trophoblasts by inducible nitric oxide synthase promotes trophoblast invasion[J].Exp Cell Res,2008,314(8):1765-1776.
[8]	GESSLER P,BUCHAL P,SCHWENK H U,et al.Favourable long-term outcome after immediate treatment of neonatal hyperammonemia due to N-acetylglutamate synthase deficiency[J].Eur J Pediatr,2010,169(2):197-199.
[9]	MORIZONO H,CALDOVIC L,SHI D S,et al.Mammalian N-acetylglutamate synthase[J].Mol Genet Metab,2004,81 Suppl 1(S1):4-11.
[10]	ZHU J L,ZENG X F,PENG Q,et al.Maternal N-carbamylglutamate supplementation during early pregnancy enhances embryonic survival and develop-ment through modulation of the endometrial proteome in gilts[J].J Nutr,2015,145(10):2212-2220.
[11]	CAI S,ZHU J L,ZENG X Z,et al.Maternal N-carbamylglutamate supply during early pregnancy enhanced pregnancy outcomes in sows through modulations of targeted genes and metabolism pathways[J].J Agric Food Chem,2018,66(23):5845-5852.
[12]	江雪梅,吴德,方正锋,等.饲粮添加L-精氨酸或N-氨甲酰谷氨酸对经产母猪繁殖性能及血液参数的影响[J].动物营养学报,2011,23(7):1185-1193.JIANG X M,WU D,FANG Z F,et al.Effects of dietary L-arginine or N-carbamylglutamate on reproductive performance and blood parameters of multiparous sows[J].Chinese Journal of Animal Nutrition,2011,23(7):1185-1193.(in Chinese)
[13]	孙玲伟,王智博,安世钰,等.RP-Arg和NCG对营养限饲湖羊胎盘发育的影响[J].南京农业大学学报,2020,43(1):125-133.SUN L W,WANG Z B,AN S Y,et al.Effects of dietary RP-Arg and NCG supplementation on development of maternal and fetal placenta in nutrient restriction Hu sheep during pregnancy[J].Journal of Nanjing Agricultural University,2020,43(1):125-133.(in Chinese)
[14]	SUN L W,ZHANG H,WANG Z Y,et al.Dietary rumen-protected arginine and N-carbamylglutamate supplementation enhances fetal growth in underfed ewes[J].Reprod Fertil Dev,2018,30(8):1116-1127.
[15]	ZHANG H,SUN L W,WANG Z Y,et al.Dietary N-carbamylglutamate and rumen-protected L-arginine supplementation ameliorate fetal growth restriction in undernourished ewes[J].J Anim Sci,2016,94(5):2072-2085.
[16]	WU G Y,BAZER F W,CUDD T A,et al.Maternal nutrition and fetal development[J].J Nutr,2004,134(9):2169-2172.
[17]	WU G Y,POND W G,OTT T,et al.Maternal dietary protein deficiency decreases amino acid concentrations in fetal plasma and allantoic fluid of pigs[J].J Nutr,1998,128(5):894-902.
[18]	CHACHER B,LIU H Y,WANG D M,et al.Potential role of N-carbamoyl glutamate in biosynthesis of arginine and its significance in production of ruminant animals[J].J Anim Sci Biotechnol,2013,4(1):16.
[19]	KIM J Y,BURGHARDT R C,WU G Y,et al.Select nutrients in the ovine uterine lumen.VII.Effects of arginine,leucine,glutamine,and glucose on trophectoderm cell signaling,proliferation,and migration[J].Biol Reprod,2011,84(1):62-69.
[20]	KIM J Y,BURGHARDT R C,WU G Y,et al.Select nutrients in the ovine uterine lumen.VIII.Arginine stimulates proliferation of ovine trophectoderm cells through MTOR-RPS6K-RPS6 signaling cascade and synthesis of nitric oxide and polyamines[J].Biol Reprod,2011,84(1):70-78.
[21]	LASSALA A,BAZER F W,CUDD T A,et al.Parenteral administration of L-arginine prevents fetal growth restriction in undernourished ewes[J].J Nutr,2010,140(7):1242-1248.
[22]	LASSALA A,BAZER F W,CUDD T A,et al.Parenteral administration of L-arginine enhances fetal survival and growth in sheep carrying multiple fetuses[J].J Nutr,2011,141(5):849-855.
[23]	刘星达,吴信,印遇龙,等.妊娠后期日粮中添加不同水平N-氨甲酰谷氨酸对母猪繁殖性能的影响[J].畜牧兽医学报,2011,42(11):1550-1555.LIU X D,WU X,YIN Y L,et al.Effects of different dietary N-carbamylglutamate supplementation on the reproductive performance of sows during late pregnancy[J].Acta Veterinaria et Zootechnica Sinica,2011,42(11):1550-1555.(in Chinese)
[24]	BIRD I M,ZHANG L B,MAGNESS R R.Possible mechanisms underlying pregnancy-induced changes in uterine artery endothelial function[J].Am J Physiol Regul Integr Comp Physiol,2003,284(2):R245-R258.
[25]	HANSSON S R,NÄÄV Å,ERLANDSSON L.Oxidative stress in preeclampsia and the role of free fetal hemoglobin[J].Front Physiol,2015,5:516.
[26]	WU G Y,BAZER F W,DAVIS T A,et al.Important roles for the arginine family of amino acids in swine nutrition and production[J].Livest Sci,2007,112(1-2):8-22.
[27]	SHALABY F,ROSSANT J,YAMAGUCHI T P,et al.Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice[J].Nature,1995,376(6535):62-66.
[28]	PARENTI A,MORBIDELLI L,CUI X L,et al.Nitric oxide is an upstream signal of vascular endothelial growth factor-induced extracellular signal-regulated kinase activation in postcapillary endothelium[J].J Biol Chem,1998,273(7):4220-4226.
[29]	SHIZUKUDA Y,TANG S Q,YOKOTA R,et al.Vascular endothelial growth factor-induced endothe-lial cell migration and proliferation depend on a nitric oxide-mediated decrease in protein kinase Cδ activity[J].Circ Res,1999,85(3):247-256.
[30]	ZHENG J,WEN Y X,AUSTIN J L,et al.Exogenous nitric oxide stimulates cell proliferation via activation of a mitogen-activated protein kinase pathway in ovine fetoplacental artery endothelial cells[J].Biol Reprod,2006,74(2):375-382.
[31]	GIEN J,SEEDORF G J,BALASUBRAMANIAM V,et al.Intrauterine pulmonary hypertension impairs angiogenesis in vitro:role of vascular endothelial growth factor-nitric oxide signaling[J].Am J Respir Crit Care Med,2007,176(11):1146-1153.
[32]	BUSTAMANTE S A,PANG Y,ROMERO S,et al.Inducible nitric oxide synthase and the regulation of central vessel caliber in the fetal rat[J].Circulation,1996,94(8):1948-1953.
[33]	LIU X D,WU X,YIN Y L,et al.Effects of dietary L-arginine or N-carbamylglutamate supplementation during late gestation of sows on the miR-15b/16,miR-221/222,VEGFA and eNOS expression in umbilical vein[J].Amino Acids,2012,42(6):2111-2119.
[34]	NEWSHOLME P,BRENNAN L,RUBI B,et al.New insights into amino acid metabolism,β-cell function and diabetes[J].Clin Sci (Lond),2005,108(3):185-194.
[35]	朱士恩.家畜繁殖学[M].6版.北京:中国农业出版社,2015:47.ZHU S E.Animal reproduction[M].6th ed.Beijing:China Agriculture Press,2015:47.(in Chinese)
[36]	CHACHER B,ZHU W,YE J A,et al.Effect of dietary N-carbamoylglutamate on milk production and nitrogen utilization in high-yielding dairy cows[J].J Dairy Sci,2014,97(4):2338-2345.
[37]	TUCHMAN M,CALDOVIC L,DAIKHIN Y,et al.N-carbamylglutamate markedly enhances ureagenesis in N-acetylglutamate deficiency and propionic acidemia as measured by isotopic incorporation and blood biomarkers[J].Pediatr Res,2008,64(2):213-217.

妊娠早期饲粮添加不同水平N-氨甲酰谷氨酸改善母羊产羔性能的研究

Effects of Dietary N-carbamylglutamate Supplementation on Improving Lambing Performance of Ewes during Early Pregnancy

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