饲喂PRL抑制剂对燕山绒山羊毛囊发育的影响

doi:10.11843/j.issn.0366-6964.2021.012.016

摘要/Abstract

摘要： 旨在研究绒山羊毛囊生长期饲喂催乳素（PRL）抑制剂对毛囊发育的影响。本研究选择3月龄体重相近（（23.01±4.82） kg）、健康良好的燕山绒山羊公羊20只，随机分为两组，每组10只，分别为对照组和试验组，试验组饲喂PRL抑制剂（0.06 mg·kg^-1），试验期90 d。试验结束时采集羊绒纤维、血液和皮肤样品，分析饲喂PRL抑制剂对山羊绒长度、细度、血液指标、毛囊性状及皮肤组织PRL、MTNR1a和RORα mRNA表达水平的影响。结果表明，饲喂PRL抑制剂显著提高了绒山羊初级毛囊数量、次级毛囊数量和S/P值（P<0.05），并显著提高了活性初级毛囊占比和活性次级毛囊占比（P<0.05），对山羊绒伸直长度和细度无显著影响（P>0.05）。饲喂PRL抑制剂3个月对绒山羊血清中PRL、MT、IGF-1、COR、GH、T₄和T₃均无显著影响（P>0.05），但显著降低了皮肤中PRL和MTNR1a mRNA表达水平（P<0.05），对RORα mRNA表达量无显著影响（P>0.05）。综上，在绒山羊毛囊生长期抑制PRL分泌可能是通过降低皮肤中PRL和MTNR1a基因的表达来促进毛囊发育。

关键词: 绒山羊, PRL, 次级毛囊, 血液指标, MTNR1a

Abstract: This experiment was conducted to study the effect of prolactin (PRL) inhibitor on hair follicle development of cashmere goats during anagen. Twenty healthy Yanshan cashmere goats with similar body weight ((23.01±4.82)kg) aged 3 months old were selected. Goats were randomly divided into two groups with 10 goats in each group, which were control group and experimental group, respectively. The goats in experimental group were fed PRL inhibitor (0.06 mg·kg^-1) for 90 days. At the end of the experiment, cashmere fiber, blood and skin samples were collected to analyze the effects of PRL inhibitor on the length and fineness of cashmere, blood indexes, hair follicle traits and the expression levels of PRL, MTNR1a and RORα mRNA in skin tissues. The results indicated that the length and diameter of fibers were not significantly influenced (P>0.05) by the PRL inhibitor. The PRL inhibitor significantly increased primary and secondary hair follicle numbers and the ratio of secondary number to primary number (S/P,P<0.05), and significantly increased the percentage of active primary and secondary hair follicles in anagen (P<0.05). The PRL inhibitor had no significant effect on the serum concentrations of PRL, melatonin (MT), insulin-like growth factor-1(IGF-1), cortisol (COR), growth hormone (GH), tetraiodothyronine (T₄) and triiodothyronine (T₃), but significantly decreased mRNA expressions of PRL and MTNR1a in skin (P<0.05), and had no significant effect on mRNA expression of RORα (P>0.05). Inhibition of PRL secretion during the anagen phase of goat hair follicle may promote hair follicle development by reducing the expression of PRL and MTNR1a genes in the skin.

Key words: cashmere goat, PRL, secondary hair follicle, blood indicator, MTNR1a

中图分类号:

S827.4

张乐超, 段春辉, 刘月琴, 张英杰. 饲喂PRL抑制剂对燕山绒山羊毛囊发育的影响[J]. 畜牧兽医学报, 2021, 52(12): 3492-3500.

ZHANG Lechao, DUAN Chunhui, LIU Yueqin, ZHANG Yingjie. Effect of PRL Inhibitor on Hair Follicle Development of Yanshan Cashmere Goat[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3492-3500.

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