1株重组猪繁殖与呼吸综合征病毒的基因组特征

doi:10.11843/j.issn.0366-6964.2020.12.027

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (12): 3181-3186.doi: 10.11843/j.issn.0366-6964.2020.12.027

1株重组猪繁殖与呼吸综合征病毒的基因组特征

谭祥梅¹, 吴瑕¹, 陈鹏飞¹, 赵雄伟¹, 周书亭^1,2, 虞凌雪¹, 童武¹, 高飞¹, 姜一峰¹, 于海¹, 童光志^1,3, 周艳君^1,3*

1. 中国农业科学院上海兽医研究所, 上海 200241;
2. 上海交通大学农业与生物学院, 上海 200240;
3. 江苏省动物重要疫病与人兽共患病防控协同创新中心, 扬州 225009

收稿日期:2020-05-13 出版日期:2020-12-25 发布日期:2020-12-23
通讯作者: 周艳君,主要从事动物病毒学研究,E-mail:yjzhou@shvri.ac.cn
作者简介:谭祥梅(1995-),女,重庆人,硕士生,主要从事猪繁殖与呼吸综合征病毒学研究,E-mail:txiangmei6@163.com
基金资助:
国家重点研发计划项目（2017YFD0501104）；上海市科技兴农项目（2020-02-08-00-08-F01465）；国家自然科学基金（31702240）；国家生猪现代产业技术体系（CARS-35）

Genome Characterization of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus

TAN Xiangmei¹, WU Xia¹, CHEN Pengfei¹, ZHAO Xiongwei¹, ZHOU Shuting^1,2, YU Lingxue¹, TONG Wu¹, GAO Fei¹, JIANG Yifeng¹, YU Hai¹, TONG Guangzhi^1,3, ZHOU Yanjun^1,3*

1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China;
2. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China;
3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China

Received:2020-05-13 Online:2020-12-25 Published:2020-12-23

摘要/Abstract

摘要： 猪繁殖与呼吸综合征病毒（PRRSV）不断发生变异，其防控难度也逐渐增加。为了及时监测PRRSV在自然感染背景下的遗传进化特点，本研究从上海某发病猪场的临床样品中分离获得1株PRRSV毒株，命名为SHpd1/2018株，并对其生物学特性和基因组序列进行分析。结果表明，该毒株基因组全长为15 018 bp（不含poly A），不能被针对HP-PRRSV强毒HuN4株的Nsp2的单抗所识别，但其增殖特性与强毒HuN4株相似。序列分析表明，SHpd1/2018株与HP-PRRSV强毒株的相似性最高（与HuN4相似性为94.3%）。重组分析结果显示，SHpd1/2018株是以HP-PRRSV-like为主要亲本毒株，NADC30-like为次要亲本毒株的重组病毒，两个重组断点nt 2002和nt 3205均位于Nsp2基因的高变区。研究证实SHpd1/2018株是1株发生变异重组的PRRSV分离株，该毒株是上海某猪场保育猪发病的主要原因。

关键词: 猪繁殖与呼吸综合征病毒, SHpd1/2018, 生物学特性, 基因重组分析

Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) mutates continuously, and it is increasingly difficult to control it. To monitor the genetic evolution characteristics of PRRSV timely under the condition of natural infection, a novel PRRSV variant named SHpd1/2018 was isolated from PRRSV positive clinical samples. The results showed that the SHpd1/2018, with a total genome length of 15 018 bp (excluding poly A), showed similar proliferation characteristics to HP-PRRSV strain HuN4. However, it could not be recognized by the monoclonal antibody against HuN4-Nsp2. The sequence analysis indicated that SHpd1/2018 had the greatest homology with HP-PRRSV-like strains, reaching 94.3% with HuN4 strain. The results of recombination analysis showed that SHpd1/2018 was recombined from HP-PRRSV-like strain (main parent strain) and NADC30 strain (secondary parent strain), and both the two recombination breakpoints nt2002 and nt3205 are located in the hypervariable regions of the Nsp2 gene. In short, we confirmed that the isolated strain SHpd1/2018 is a recombinant PRRSV, which may be the main cause of the disease in the pig farm in Shanghai.

Key words: porcine reproductive and respiratory syndrome virus, SHpd1/2018, phylogenetic analysis, recombination analysis

中图分类号:

S852.659.6

谭祥梅, 吴瑕, 陈鹏飞, 赵雄伟, 周书亭, 虞凌雪, 童武, 高飞, 姜一峰, 于海, 童光志, 周艳君. 1株重组猪繁殖与呼吸综合征病毒的基因组特征[J]. 畜牧兽医学报, 2020, 51(12): 3181-3186.

TAN Xiangmei, WU Xia, CHEN Pengfei, ZHAO Xiongwei, ZHOU Shuting, YU Lingxue, TONG Wu, GAO Fei, JIANG Yifeng, YU Hai, TONG Guangzhi, ZHOU Yanjun. Genome Characterization of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 3181-3186.

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1株重组猪繁殖与呼吸综合征病毒的基因组特征

Genome Characterization of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus

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