翠雀内生真菌分离鉴定及分布特性研究

doi:10.11843/j.issn.0366-6964.2020.09.028

摘要/Abstract

摘要： 旨在探明有毒植物翠雀的内生真菌种类，作者采用表面消毒法分离，运用形态学鉴定，采用ITS序列构建系统进化树，确定了其内生真菌的种类。结果显示，从翠雀植物中分离得到22种内生真菌，将其根据亲缘关系进行系统进化分析，主要分为Ⅰ和Ⅱ两大种群，分属于6纲、6目、6科、7属，其中，链格孢属真菌（Alternaria sp.）为优势菌（占分离菌株数的31.82%，主要分布于花和叶中），内生真菌在花中分布最普遍（占分离菌株数的40.91%），其次，为叶（31.82%）、根（22.73%）和茎（4.55%）。结果表明，翠雀内生真菌在不同组织中的分布普遍性不同。

关键词: 翠雀, 内生真菌, 分离鉴定, 分布特性, 链格孢菌属

Abstract: In order to explore the species of endophytic fungi from a poisonous plant, Delphinium grandiflorum L. In this experiment, surface disinfection was used for separation, morphological identification was used, and phylogenetic tree was constructed using ITS sequence to determine the species of endophytic fungi. The results showed that 22 species of endophytic fungi were isolated from D. grandiflorum L., they were mainly divided into two groups according to their phylogenetic relationship, belonging to 6 classes, 6 orders, 6 families, and 7 genera. Among them, Alternaria sp. was the dominant species (accounting for 31.82% of the isolated strains, mainly distributed in flowers and leaves), endophytic fungi are most commonly distributed in flowers (accounting for 40.91% of the isolates), followed by leaves (31.82%), roots (22.73%) and stems (4.55%). The results indicated that the distribution of endophytic fungi in D. grandiflorum L. is different in different tissues.

Key words: Delphinium grandiflorum L., endophytic fungus, isolation and identification, distribution characteristics, Alternaria sp.

中图分类号:

S856.9
S452

朱燕丽, 李博, 张雨, 孙璐, 黄恩霞, 莫重辉, 赵宝玉, 路浩. 翠雀内生真菌分离鉴定及分布特性研究[J]. 畜牧兽医学报, 2020, 51(9): 2302-2311.

ZHU Yanli, LI Bo, ZHANG Yu, SUN Lu, HUANG Enxia, MO Chonghui, ZHAO Baoyu, LU Hao. Isolation, Identification and Distribution Characteristics of Fungal Endophyte in Delphinium grandiflorum L.[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2302-2311.

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