Apelin-13对小鼠血脂代谢的影响及其机制研究

doi:10.11843/j.issn.0366-6964.2020.09.018

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (9): 2207-2215.doi: 10.11843/j.issn.0366-6964.2020.09.018

Apelin-13对小鼠血脂代谢的影响及其机制研究

张敏^1,2,3, 李建磊¹, 朱雨婷¹, 陈羽¹, 林泽¹, 吴琼^1,2,3, 李焰^1,2,3, 孙艳发^1,2,3*

1. 龙岩学院生命科学学院, 龙岩 364012;
2. 福建省家畜传染病防治与生物技术重点实验室, 龙岩 364012;
3. 预防兽医学与生物技术福建省高校重点实验室, 龙岩 364012

收稿日期:2019-12-09 出版日期:2020-09-25 发布日期:2020-09-25
通讯作者: 孙艳发,主要从事家禽营养与遗传育种研究,E-mail:boysun2010@163.com
作者简介:张敏(1983-),女,黑龙江尚志人,博士,讲师,主要从事动物传染病病原检测研究,E-mail:zhangminshiwo@163.com
基金资助:
国家自然科学基金（31501970）；福建省自然科学基金（2017J01640）；福建省高校杰出青年科研人才培育计划；杨胜先生门生社群项目（C2016046）；福建省大学生创新创业训练计划项目（201811312052）

Study of Apelin-13 on Blood Lipid Metabolism and Its Mechanism in Mice

ZHANG Min^1,2,3, LI Jianlei¹, ZHU Yuting¹, CHEN Yu¹, LIN Ze¹, WU Qiong^1,2,3, LI Yan^1,2,3, SUN Yanfa^1,2,3*

1. College of Life Science, Longyan University, Longyan 364012, China;
2. Key Laboratory of Fujian Province Livestock Epidemic Prevention and Control and Biological Technology, Longyan 364012, China;
3. Key Laboratory of Fujian Universities Preventive Veterinary Medicine and Biotechnology(Longyan University), Longyan 364012, China

Received:2019-12-09 Online:2020-09-25 Published:2020-09-25

摘要/Abstract

摘要： 本研究旨在探索Apelin-13调控小鼠血脂代谢的生理机制。试验选取体重相近的小鼠96只，随机分为4个处理，每个处理4个重复。对照组和3个试验组小鼠分别每天皮下注射100 μL 0.9%的生理盐水、10、20和50 μg·kg^-1的Apelin-13，试验期为14 d。测定小鼠的生长性能、血清Apelin-13和血脂含量，并采用Illumina Novaseq测序平台进行小鼠肝组织转录组测序（RNA-seq）。结果表明，与对照组相比，皮下注射Apelin-13极显著提高了血清Apelin-13和高密度蛋白胆固醇含量（P<0.01），显著降低了血清甘油三酯含量（P<0.05）。试验组和处理组共检测到340个上调和350个下调的差异表达基因（differentially expressed genes，DEGs）。这些DEGs最显著富集的信号通路为视黄醇代谢，该通路中的细胞色素P450（Cyp）和尿苷二磷酸葡萄糖醛酸转移酶（Ugt）两个超家族基因大部分上调表达。试验结果表明，Apelin-13可能通过调控视黄醇代谢信号通路中的Cyp和Ugt两个超家族基因的表达来调控小鼠的血脂代谢。

关键词: 小鼠, Apelin-13, 视黄醇代谢, DEGs, 肝, RNA-seq

Abstract: The objective of this study was to explore the physiological mechanism of Apelin-13 regulating blood lipid metabolism in mice. Ninety-six mice with similar body weight were randomly divided into 4 treatments, with 4 replicates each. In a 14-day trial period, the mice of the control group and 3 treatment groups were injected subcutaneously with 100 μL 0.9% normal saline, 10, 20 and 50 μg·kg^-1 Apelin-13 each day, respectively. The growth performance, serum Apelin-13 and blood lipids contents were measured. The transcriptomic profiling of liver tissues was sequenced using Illumina Novaseq technology. The results showed that compared to control group, the serum Apelin-13 and high-density lipoprotein cholesterol contents were increased extremely significantly (P<0.01) and triglycerides contents were decreased significantly (P<0.05) in treatment groups. A total of 340 up-regulated and 350 down-regulation differentially expressed genes (DEGs) were identified among treatment groups and control group. The most significant enriched signaling pathway was retinol metabolism, in which cytochrome P450 (Cyp) and uridine diphosphate glucuronidase (Ugt) superfamily genes were mostly up-regulated. The results of this study indicated that Apelin-13 might regulate blood lipid metabolism in mice by regulating the expression of Cyp and Ugt superfamily genes in the retinol metabolism signaling pathway.

Key words: mice, Apelin-13, retinol metabolism, DEGs, liver, RNA-seq

中图分类号:

Q493.5

张敏, 李建磊, 朱雨婷, 陈羽, 林泽, 吴琼, 李焰, 孙艳发. Apelin-13对小鼠血脂代谢的影响及其机制研究[J]. 畜牧兽医学报, 2020, 51(9): 2207-2215.

ZHANG Min, LI Jianlei, ZHU Yuting, CHEN Yu, LIN Ze, WU Qiong, LI Yan, SUN Yanfa. Study of Apelin-13 on Blood Lipid Metabolism and Its Mechanism in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2207-2215.

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Apelin-13对小鼠血脂代谢的影响及其机制研究

Study of Apelin-13 on Blood Lipid Metabolism and Its Mechanism in Mice

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