玻璃化冷冻牛GV卵母细胞全基因组甲基化模式初探

doi:10.11843/j.issn.0366-6964.2020.05.014

摘要/Abstract

摘要： 旨在研究玻璃化冷冻对牛GV期卵母细胞全基因组甲基化的影响。本研究收集新鲜、玻璃化冷冻的牛GV卵母细胞，采用单细胞全基因组甲基化测序（ScWGBS）技术对新鲜、玻璃化法冷冻牛GV卵母细胞的全基因组甲基化水平进行检测，旨在揭示两者DNA甲基化模式的差异。结果表明，玻璃化冷冻不会对牛GV卵母细胞的全基因甲基化水平造成显著影响。基于基因本体（GO）和信号通路（KEGG）对140个差异甲基化区域（DMRs）进行分析，发现DMRs主要参与细胞发育、细胞骨架组织等功能，主要富集在PI3K-Akt信号通路、GnRH信号通路等，并筛选出与卵母细胞成熟（TSC2）、细胞骨架（NUDC）、细胞活力（MAFK）等相关的基因。上述结果，可为提高GV卵母细胞玻璃化冷冻效率奠定信息基础和研究方向。

关键词: 玻璃化冷冻, GV卵母细胞, 单细胞全基因组甲基化测序, 牛, DMR

Abstract: The aim of this study was to investigate the effect of vitrification on the genomic methylation patterns of bovine GV oocytes. Fresh and vitrified GV oocytes were collected. Single-cell whole-genome methylation sequencing(ScWGBS) technology was used to detect the whole-genome methylation levels of the fresh and vitrified bovine GV oocytes, in order to reveal the difference of DNA methylation patterns between the fresh and vitrified bovine GV oocytes. The results showed that vitrification didn't significantly affect the whole-genome methylation level of bovine GV oocytes. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) were used to analyze 140 differentially methylated regions(DMRs). It was found that DMRs were mainly involved in cell development, cytoskeleton organization, and they were mainly enriched in the PI3K-Akt and GnRH signaling pathways, and the partial related genes were screened about oocyte maturation (TSC2), cytoskeleton (NUDC), cell viability (MAFK) etc.. These results can provide the information foundation and research direction for improving the efficiency of vitrified GV oocytes.

Key words: vitrification, GV oocytes, ScWGBS, bovine, DMR

中图分类号:

S823.3

张培培, 王晶晶, 郝海生, 杜卫华, 庞云渭, 权国波, 赵善江, 邹惠影, 郝彤, 朱化彬, 赵学明. 玻璃化冷冻牛GV卵母细胞全基因组甲基化模式初探[J]. 畜牧兽医学报, 2020, 51(5): 1030-1039.

ZHANG Peipei, WANG Jingjing, HAO Haisheng, DU Weihua, PANG Yunwei, QUAN Guobo, ZHAO Shanjiang, ZOU Huiying, HAO Tong, ZHU Huabin, ZHAO Xueming. Study on Whole Genome Methylation Pattern in Vitrified Bovine GV Oocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1030-1039.

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