猪繁殖与呼吸综合征病毒对Marc145细胞自噬相关基因转录的影响及分析

doi:10.11843/j.issn.0366-6964.2018.08.013

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (8): 1687-1692.doi: 10.11843/j.issn.0366-6964.2018.08.013

猪繁殖与呼吸综合征病毒对Marc145细胞自噬相关基因转录的影响及分析

李小璟¹, 龚双燕¹, 马海强³, 刘丹³, 高健³, 彭雪³, 朱玲^1,2, 徐志文^1,2*

1. 四川农业大学动物医学院, 成都 611130;
2. 四川农业大学动物疫病与人类健康四川省重点实验室, 成都 611130;
3. 四川百诺吉科技有限公司, 绵阳 621000

收稿日期:2018-01-04 出版日期:2018-08-23 发布日期:2018-08-23
通讯作者: 徐志文,E-mail:abtcxzw@126.com
作者简介:李小璟(1993-),女,四川成都人,硕士生,主要从事动物传染病病原分子生物学研究,E-mail:xiao_jinglee@163.com
基金资助:
四川省科技支撑计划（2017NZ0038）

The Effects of Porcine Reproductive and Respiratory Syndrome Virus on the Expression of Autophagy-Related Genes in Marc145 Cells

LI Xiao-jing¹, GONG Shuang-yan¹, MA Hai-qiang³, LIU Dan³, GAO Jian³, PENG Xue³, ZHU Ling^1,2, XU Zhi-wen^1,2*

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;
3. Sichuan Bainuoji Technology Co., Ltd, Mianyang 621000, China

Received:2018-01-04 Online:2018-08-23 Published:2018-08-23

摘要/Abstract

摘要：

自噬参与多种疾病过程，且与病毒感染与增殖关系密切。为探究Marc145细胞感染猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）后自噬相关基因Beclin1、ATG5和ATG12 mRNA转录差异及规律、PRRSV增殖与细胞自噬的联系；采用实时荧光定量PCR方法对自噬相关基因Beclin1、ATG5和ATG12 mRNA的转录进行测定，绘制了转录规律曲线图；并经实时荧光定量测定并绘制PRRSV在Marc145细胞中增殖规律图。结果显示，试验组自噬相关基因转录量明显高于对照组，表明PRRSV能诱导Marc145细胞自噬，且使Beclin1、ATG5和ATG12 mRNA提前进入高转录状态；其转录规律与细胞病变、PRRSV增殖规律呈正相关。

Abstract:

Autophagy is involved in a variety of disease processes and is closely related to viral infection and proliferation. In order to explore the difference and regularity of the mRNA expression of autophagy-related genes Beclin1, ATG5, ATG12 in Marc145 cells infected with porcine reproductive and respiratory syndrome virus (PRRSV), and to correlate PRRSV proliferation with autophagy, the real-time fluorescence quantitative PCR was used. The expressions of Beclin1, ATG5 and ATG12 mRNA were detected by real-time fluorescence quantitative PCR. The proliferation of PRRSV in Marc145 cells was measured by real-time fluorescence. The results showed that the expression of autophagy-related genes in the test group were higher than those in the control group, which means that PRRSV could induce autophagy of Marc145 cells. Meanwhile, the expression of Beclin1, ATG5 and ATG12 mRNA were advanced into the high expression state; the expression principle was positively correlated with the cytopathic effect and PRRSV proliferation.

中图分类号:

S852.659.6

李小璟, 龚双燕, 马海强, 刘丹, 高健, 彭雪, 朱玲, 徐志文. 猪繁殖与呼吸综合征病毒对Marc145细胞自噬相关基因转录的影响及分析[J]. 畜牧兽医学报, 2018, 49(8): 1687-1692.

LI Xiao-jing, GONG Shuang-yan, MA Hai-qiang, LIU Dan, GAO Jian, PENG Xue, ZHU Ling, XU Zhi-wen. The Effects of Porcine Reproductive and Respiratory Syndrome Virus on the Expression of Autophagy-Related Genes in Marc145 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1687-1692.

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猪繁殖与呼吸综合征病毒对Marc145细胞自噬相关基因转录的影响及分析

The Effects of Porcine Reproductive and Respiratory Syndrome Virus on the Expression of Autophagy-Related Genes in Marc145 Cells

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