Abstract: In this study the rabbit immunity model against Haemaphysalis longicornis was constructed by infestation with H. longicornis adult quantitatively every two weeks. The results of ELISA showed that the antibody was positive three weeks post the first infection, the antibody level rising with the increasing of infection and reached the peak at the 11th week. The resistance of rabbit against H. longicornis significantly influenced the H. longicornis plump-blooding period. The antibody level was positively correlated to the bloodsucking period and the mortality of female H. longicornis adult and negatively correlated to the weight of plumpblooding female adult. These results indicated that the salivary gland is one of the main organs in the anti-H. longicornis immunity. The maintenance of the antibody level was long enough to make H. longicornis to complete a generation, which satisfied the experiment requirement. So it is an ideal model for anti-H. longicornis immunity positive control research. At the same time, the specific antibody level in the serum can be one of the important indexes to evaluate the extent of animal resistance to H. longicornis.

Key words: Haemaphysalis longicornis, adult, immunity model, growth