ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (1): 72-78.

• 预防兽医 • Previous Articles     Next Articles

Phylogenetic Relationship of Cysticercus cellulosae Isolates in Henan Based on cox Ι Gene

ZHAO Guang-hui;ZHANG Gai-ping;NING Chang-shen;WANG Xuan-nian; ZHANG Long-xian;JIAN Fu-chun;LU Kun   

  1. 1.College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, China; 2. Henan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 3. College of Animal Science, Henan Institute of Science and Technology, Xinxiang 453003, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-01-24 Published:2008-01-24

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Abstract: 344 bp partial sequences of cox Ι gene were obtained from ten Cysticercus cellulosae isolates derived from several areas of Henan Province by RT-PCR. PCR products were cloned into pMD19-T vector and recombinant plasmids were sequenced. Nucleotide sequences of partial coxⅠgene were aligned using the ClustalX 1.81, MP and NJ trees were constructed using the software PAUP version 4.0, and ML tree was also constructed using PUZZLE version 5.2. Sequence homology analysis was performed using the software WDANSIST version 2.5 and the Megalign program of the software DNAStar version 5.0. Results indicated that there was no sequence difference among the coxⅠ gene partial sequences of ten Cysticercus cellulosae isolates, which belonging to Asian genotype. cox Ι partial sequence of Cysticercus cellulosae can be helpful to distinguish validly between Asian and American/African genetype, and can be used for differential diagnosis of different Taeniidae tapeworm. Therefore, cox Ι is expected to be a differential marker for PCR differential diagnosis for taeniasis and cysticercosis.

Key words: Cysticercus cellulosae, cox Ⅰ gene, phylogenetic relationship