7S β-伴大豆球蛋白通过NF-κB信号通路引起IPEC-J2细胞的炎性反应

doi:10.11843/j.issn.0366-6964.2019.04.020

Abstract

Abstract:

In this study, IPEC-J2 cells (porcine intestinal epithelial cells) were cultured in vitro to study the effect of different concentrations of 7S β-conglycinin on IPEC-J2. The experiment was designed as six groups:A, B, C, D, E, and F, A was the control group, and the other groups were added 1, 5, 10, 5 and 5 mg·mL^-1β-conglycinin, respectively, and 1 μmol·L^-1 NF-κB(PDTC) and iNOS (L-NAME) inhibitors were added to groups E and F, respectively. Cell viability was measured by CCK-8 method, the contents of NO, DAO, 5-HT, IL-6 and IL-10 were detected by ELISA, p-NF-κB, p65, iNOS and COX-2 protein expression levels were detected by Western blot, the relative transcription of NF-κB p65, IKKβ, iNOS, IKKα and COX-2 mRNA were determined by qPCR. The results showed that β-conglycinin reduced the viability of IPEC-J2 cells, promoted the secretion of NO, 5-HT and IL-6, decreased the secretion of IL-10, and increased the expression of p-NF-κB p65, iNOS and COX-2 proteins, transcription of NF-κB p65, IKKβ, iNOS, IKKα and COX-2 mRNA, PDTC and L-NAME can inhibit the effects of β-conglycinin. The results showed that β-conglycinin caused damage to IPEC-J2 and increased the damage with increasing concentration, PDTC and L-NAME can reduce the damage of β-conglycinin to cells.

Key words: β-conglycinin, IPEC-J2, NF-κB, PDTC, L-NAME

CLC Number:

Q251

PENG Chenglu, ZHANG Yu, DING Xuedong, LI Yu, FENG Shibin, WANG Xichun, LI Jinchun, WU Jinjie. 7S β-conglycinin Triggered Inflammatory Response in IPEC-J2 Cells through NF-κB Signaling Pathway[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 870-878.

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7S β-conglycinin Triggered Inflammatory Response in IPEC-J2 Cells through NF-κB Signaling Pathway

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