术芩提取液对脂多糖损伤的IPEC-J2细胞增殖及炎症因子转录的影响

doi:10.11843/j.issn.0366-6964.2019.07.020

Abstract

Abstract: The objective of this study was to investigate the effect of Zhu Qin Extractive Fluid (ZQEF) on the proliferation and transcription of inflammatory factors in IPEC-J2 cells injured by lipopolysaccharide (LPS). Principal active component were identified by TLC and the content of baicalin was determined by HPLC in ZQEF. IPEC-J2 cells were randomly divided into control group, LPS model group and drug group Ⅰ, Ⅱ, Ⅲ, Ⅳ, and Ⅴ (concentration of ZQEF in each group was 10^-1, 10^-2, 10^-3, 10^-4, 10^-5 g·mL^-1, respectively), each group set up 5 holes, 10⁴ cells·hole^-1. The proliferative rate of cells were determined by MTT method, and the mRNA transcription changes of TFF3, TNF-α and IL-8 were measured by qRT-PCR. The results showed:the main therapeutic ingredients include baicalin, astragaloside and atractylenolide-Ⅰ, and the content of baicalin was 1.469 8% in ZQEF. Compared with the control group, the proliferative rate of cells highly decreased, and the mRNA transcription of TFF3, TNF-α and IL-8 extremely increased in LPS model group (P<0.01). Compared with the LPS model group, the proliferative rate of cells highly enhanced in drug group Ⅱ (10^-2g·mL^-1) and Ⅲ (10^-3 g·mL^-1) (P<0.01 and P<0.05 respectively), the mRNA transcription of TFF3 and TNF-α significantly down-regulated (P<0.01), and the mRNA transcription of IL-8 down-regulated in drug group Ⅱ, non-significantly (P>0.05). The results suggest that ZQEF could facilitate the proliferation of IPEC-J2 cells injured by LPS, inhibit the transcription of inflammatory factors, which may be connected with effect of repairing the intestinal mucosa.

CLC Number:

S853.74

WANG Zhongqing, LIN Chunfa, ZHONG Wenjie, LUO Yichen, ZHU Zhaorong, LIU Juan. Effect of Zhu Qin Extractive Fluid on the Proliferation and Transcription of Inflammatory Factors in LPS-injured IPEC-J2 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1500-1508.

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Effect of Zhu Qin Extractive Fluid on the Proliferation and Transcription of Inflammatory Factors in LPS-injured IPEC-J2 Cells

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