犬乳腺肿瘤裸鼠模型建立及其生长特性

doi:10.11843/j.issn.0366-6964.2019.04.019

Abstract

Abstract:

This research was conducted to establish a nude mice model of canine mammary cancer cell,and study its biological characteristics as well as dynamic changes. The green fluorescent protein (GFP) was introduced into the CHMm and CHMp cells of canine breast cancer cell. The nude mice model was established by implantation of tumor cell suspension in right second breast of the nude mice. The dynamic changes of tumor in nude mice at different time periods were studied by the technique of living fluorescence imaging and pathological examination. Results were as follows:GFP labeled CHMm-GFP and CHMp-GFP cells were observed to emit stable green fluorescence signals under inverted fluorescence microscope, and macroscopic tumor appeared on the 6th day after implantation. The tumor growth curve and weight change curve were drawn by fluorescence signal detection and pathological examination on the 25th day and the 60th day after implantation. On the 25th day after implantation, the fluorescence signal of CHMm-GFP was the strongest in nude mice, while that of CHMp-GFP was strongest on 35th day. On the 25th day after implantation, it was found that one lymph node of each group of nude mice showed fluorescence signal. On the 35th day, fluorescent signals were displayed in lymph nodes of 3 nude mice, but no fluorescence signals were found in other organs. On the 25th day, one nude mouse tumor metastasized to the lymph node, and at the 35th day and the 60th day, the tumor was found to have metastasized to the lymph node. Canine breast tumor models of two cell lines were established in this study. The two cell lines showed different growth characteristics in nude mice at different periods and could be metastasized, which provided a reference for the further study of the occurrence and development of mammary tumors.

Key words: canine mammary cancer cell, tumor model, nude mice, growth characteristics

CLC Number:

S857.26

LIU Zhen, RAO Jiarong, Lü Junjin, GOU Si, LI Huizhen, LIN Hui, YUAN Liguo. Establishment of a Nude Mouse Model of Canine Mammary Tumor and Its Growth Characteristics[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 861-869.

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Establishment of a Nude Mouse Model of Canine Mammary Tumor and Its Growth Characteristics

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[1]	LIU Zhen, RAO Jiarong, Lü Junjin, GOU Si, LI Huizhen, LIN Hui, YUAN Liguo. Establishment of a Nude Mouse Model of Canine Mammary Tumor and Its Growth Characteristics [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 861-869.
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