黄芪甲苷对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞NO、ET-1的影响

doi:10.11843/j.issn.0366-6964.2019.04.021

Abstract

Abstract:

The study was conducted to investigate the effects of astragaloside (Ast) on microvascular vasomotion at Hegu acupoint, nitric oxide and endothelin-1 released by rat myocardial microvascular endothelial cells (RMMECs). Laser Doppler flowmetry combined with iontophoresis was used to detect the amplitude of microvascular vasomotion. ELISA method was used to detect the levels of NO and ET-1 released by RMMECs incubated with different doses of Ast for 3, 6, 9 and 12 hours. Real-Time PCR was used to detect the gene transcription of NO and ET-1 in RMMECs treated with 10 μg·mL^-1Ast. The results were as follows:After introduction of Ast, the amplitude of microvascular vasomotion at Hegu acupoint was improved. Compared with control group (RMMECs), the levels of NO and ET-1 were improved, NO/ET-1 value was increased by Ast in Ast incubated RMMECs. When RMMECs were incubated with 10 μg·mL^-1 Ast for 3 hours, the NO/ET-1 value was the highest, and the gene transcription levels of NO and ET-1 were improved, which were consistent with the results of secretion level. When 25 μg·mL^-1 Ast incubated RMMECs for 3 hours, its NO/ET-1 value was slightly lower than that of 10 μg·mL^-1 Ast group, but its ratio could be maintained at a relatively high level. The results showed that Ast could increase the amplitude of microvascular vasomotion, and its mechanism might be through regulating the dynamic balance of NO and ET-1 released by RMMECs in circulatory system.

Key words: microvascular vasomotion, astragaloside, NO, ET-1, RMMECs

CLC Number:

Q251

LI Chunxiao, LIU Xiaoyu, WANG Xin, WANG Yueming, WEI Donglai, LI Qiuyue, HE Shangwen, MU Xiang, DONG Hong. Effect of Astragaloside on Microvascular Vasomotion and Nitric Oxide, Endothelin-1 Secretion from Rat Myocardia Microvascular Endothelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 879-886.

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Effect of Astragaloside on Microvascular Vasomotion and Nitric Oxide, Endothelin-1 Secretion from Rat Myocardia Microvascular Endothelial Cells

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