牦牛源干燥棒状杆菌的分离与鉴定

doi:10.11843/j.issn.0366-6964.2019.01.026

Abstract

Abstract:

To investigate the formation reasons of caseous nodules in the liver of a yak, bacteria were isolated and cultured from the liver, and the 16S rRNA and rpoB gene sequences of isolated bacteria were amplified and sequenced. The biochemical phenotypes were identified by Vitek 2 automatic microbiology analyzer and drug susceptibility test was performed by K-B method. Results were as follows:A Gram-positive, rod-shaped and suspected pathogen was isolated. The amplification lengths of 16S rRNA and rpoB were 1 483 and 434 bp, while the accession number were MH000696 and MH006608, respectively. The similarity of 16S rRNA of clinical isolate to Corynebacterium xerosis was as high as 99% and phylogenetic tree showed it lied in the same branch with Corynebacterim xerosis. The results of Vitek 2 fully automatic Microbial Identification System showed that it was identified as Kocuria varians, with the probability of 93%. The bacteria was highly sensitive to tobramycin, aztreonam, cefoperazone, minocycline and other medicines. In summary, the isolated bacteria is Corynebacterium xerosis. But the gene sequence, such as rpoB, and biochemical property have a certain degree of mutations. This study provides reference for the isolation and identification in the veterinary clinic, and is the foundation of pathogenesis research of this bacteria.

Key words: Corynebacterium xerosis, 16S rRNA, rpoB, biochemical test, drug sensitivity test

CLC Number:

S855.1

WEN Fengqin, XIANG Haitao, HAO Baocheng, XING Xiaoyong, BAO Shijun, HU Yonghao. Isolation and Identification of Corynebacterium Xerosis Isolated from Yak Liver[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 227-232.

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Isolation and Identification of Corynebacterium Xerosis Isolated from Yak Liver

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