大熊猫源酵母菌的分离鉴定

doi:10.11843/j.issn.0366-6964.2017.12.018

Abstract

Abstract:

In order to investigate species and genus distribution of yeast on hairs of giant panda and their pathogenicity on mice, thirty-seven hair samples of giant panda were collected and 45 isolates of yeast were isolated from the samples with traditional microbial isolation method. PCR amplification on the strains using the universal fungi primer of ITS1 and ITS4 and phylogenetic analysis were performed. And the 45 isolates belonged to 12 species. Drug sensitive qualitative test of 12 species of yeast were researched using 5 kinds of clinical commonly antifungal medicines by the Danish Rosco disc diffusion method. The results indicated that 12 species of yeast were isolated, including Trichosporon cutaneum, Trichosporon moniliiforme, Trichosporon interdigitale, Trichosporon akiyoshidainum, Trichosporon asteroids, Trichosporon brassicae, Trichosporon guehoae, Trichosporon jirovecii, Trichosporon montevideense, Trichosporon sp., Candida catenulate and Cryptococcus fragicola. Most of the yeast strains were sensitive to 5 kinds of antifungal drugs. C. fragicola was resistant to ketoconazole, C. catenulate and T. interdigitale were resistant to amphotericin B, C. catenulate and T. jirovecii were resistant to itraconazole, Trichosporon sp. was resistant to fluconazole,C. catenulate, T. interdigitale, T. guehoae and T. cutaneum were resistant to nystati. T. cutaneum, T. interdigitale, T. asteroids, C. fragicola were pathogenic to mice, and other isolates of yeasts were opportunistic pathogenicity. Various yeast species exist in surface of giant panda, and they have different drug sensitivity to fungicides. These results have greatly enriched the understanding of the yeast on hair of giant panda,will provide a reference for the dermatomycoses diagnosis and treatment of giant panda.

CLC Number:

S852.66

MA Xiao-ping, XIANG Qi, WANG Cheng-dong, CAO San-jie, LING Shan-shan, GU Yu. Isolation, Identification and Drug Resisitance Analysis of Yeast from Hair of Ailuropoda melanoleuca[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2383-2391.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.12.018

https://www.xmsyxb.com/EN/Y2017/V48/I12/2383

References

[1] 四川在线. 第四次全国大熊猫调查结果:中国野生大熊猫1864只川甘陕均有分布[EB/OL]. 2015-02-28.[2017-09-14]. http://sichuan.scol.com.cn/fffy/201502/10033289.html. Sichuan Online. The fourth national giant panda survey:China has 1864 wild pandas, distribute in Sichuan, Gansu and Shanxi provinces[EB/OL]. 2015-02-28.[2017-09-14]. http://sichuan.scol.com.cn/fffy/201502/10033289.html. (in Chinese)
[2] 央视网. 全球今年繁育成活大熊猫宝宝40只人工圈养量达425只[EB/OL]. 2015-11-12.[2017-09-14]. http://m.news.cntv.cn/2015/11/12/ARTI1447286910344854.shtml. CCTV. The global giant panda breed and survive 40 babies in this year, number of artificial captive up to 425[EB/OL]. 2015-11-12.[2017-09-14]. http://m.news.cntv.cn/2015/11/12/ARTI1447286910344854.shtml. (in Chinese)
[3] 马晓平, 向奇, 李德生, 等. 大熊猫被毛可培养真菌的分离鉴定与系统发育学分析[J]. 中国兽医科学, 2017, 47(1):72-81.
MA X P, XIANG Q, LI D S, et al. Isolation, identification and phylogenetic analysis of culturable fungi in hair of Ailuropoda melanoleuca[J]. Chinese Veterinary Science, 2017, 47(1):72-81. (in Chinese)
[4] 张悦天, 马晓平, 古玉, 等. 大熊猫源石膏样小孢子菌的分离鉴定与致病性研究[J]. 中国兽医科学, 2015, 45(6):551-559.
ZHANG Y T, MA X P, GU Y, et al. Identification and pathogenicity of microsporum gypseum isolated from Ailuropoda melanoleuca[J]. Chinese Veterinary Science, 2015, 45(6):551-559. (in Chinese)
[5] 王承东, 叶娇, 李德生, 等. 一例老龄大熊猫死亡后的实验室检验与体会[J]. 畜牧与兽医, 2015, 47(3):75-76.
WANG C D, YE J, LI D S, et al. The experience on laboratory verification of an aged giant panda afterdeath[J]. Animal Husbandry and Veterinary Medicine, 2015, 47(3):75-76. (in Chinese)
[6] HINRIKSON H P, HURST S F, LOTT T J, et al. Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species[J]. J Clin Microbiol, 2005, 43(5):2092-2103.
[7] LINTON C J, BORMAN A M, CHEUNG G, et al. Molecular identification of unusual pathogenic yeast isolates by large ribosomal subunit gene sequencing:2 years of experience at the United kingdom mycology reference laboratory[J]. J Clin Microbiol, 2007, 45(4):1152-1158.
[8] 马晓平, 杨秋霞, 李长城, 等. 发情期大熊猫阴道真菌的分离鉴定及酵母菌药敏试验[J]. 中国兽医科学, 2016, 46(10):1233-1240.
MA X P, YANG Q X, LI C C, et al. Isolation and identification of fungi from the vagina of estrus giant panda and drug sensitive tests of the isolated yeasts[J]. Chinese Veterinary Science, 2016, 46(10):1233-1240. (in Chinese)
[9] PFALLER M A, DIEKEMA D J, MESSER S A, et al. In vitro activities of caspofungin compared with those of fluconazole and itraconazole against 3,959 clinical isolates of Candida. spp., including 157 fluconazole-resistant isolates[J]. Antimicrob Agents Chem other, 2003, 47(3):1068-1071.
[10] 王端礼. 医学真菌:实验室检验指南[M]. 北京:人民卫生出版社, 2005.
WANG D L. Medical mycology:Guide to laboratory examination[M]. Beijing:People's Medical Publishing House, 2005. (in Chinese)
[11] 马晓平, 古玉, 刘小敏, 等. 大熊猫源枝孢样枝孢霉的分离鉴定[J]. 中国兽医科学, 2013, 43(12):1217-1223.
MA X P, GU Y, LIU X M, et al. Isolation and identification of Cladosporium cladosporioides from Ailuropoda melanoleuca[J]. Chinese Veterinary Science, 2013, 43(12):1217-1223. (in Chinese)
[12] 曹永军, 程萍, 喻国辉, 等. 利用ITS1和ITS4通用引物扩增香蕉枯萎病菌核酸片段鉴定其生理小种[J]. 热带作物学报, 2010, 31(7):1098-1102.
CAO Y J, CHENG P, YU G H, et al. Utility of universal primers, ITS1 and ITS4, to amplify sequences for race identification of Fusarium oxysporum f. sp. cubense[J]. Chinese Journal of Tropical Crops, 2010, 31(7):1098-1102. (in Chinese)
[13] SELIGER H. PCR protocols-A guide to methods and applications[M]. New York:Academic Press, 1990.
[14] 陶仲为.隐球菌感染[J].医师进修杂志,2002,25(1):9-10.
TAO Z W. Cryptococcal pneumonia[J]. Journal of Postgraduates of Medicine, 2002, 25(1):9-10. (in Chinese)
[15] 苏丹虹,邬全会,王华成.Rosco真菌药敏试验与其他方法的比较研究[J].检验医学,2004,19(4):291-294.
SU D H, WU Q H, WANG H C. Comparative study on Rosco antifungal susceptibility test with other methods[J]. Laboratory Medicine, 2004, 19(4):291-294. (in Chinese)
[16] 张德全,夏志宽,敖俊红,等.阿萨希毛孢子菌感染治疗新进展[J].中国皮肤性病学杂志,2015,29(8):861-863.
ZHANG D Q, XIA Z K, AO J H, et al. Recent progress in anti-infective therapy of Trichosporon asahii[J]. The Chinese Journal of Dermatovenereology, 2015, 29(8):861-863. (in Chinese)
[17] CHAGAS-NETO T C, CHAVES G M, MELO A S A, et al. Bloodstream infections due to Trichosporon spp.:species distribution, Trichosporon asahii genotypes determined on the basis of ribosomal DNA intergenic spacer 1 sequencing, and antifungal susceptibility testing[J]. J Clin Microbiol, 2009, 47(4):1074-1081.
[18] 徐英春, 李若瑜, 倪语星, 等. 如何正确理解抗真菌药物体外敏感试验结果对临床用药的指导价值[J]. 中华检验医学杂志, 2008, 31(2):128-132.
XU Y C, LI R Y, NI Y X, et al. How to interpret the guidance value of in vitro susceptibility test of anti-fungal drugs on clinical practice[J]. Chinese Journal of Laboratory Medicine, 2008, 31(2):128-132. (in Chinese)
[19] 刘小平, 李建武, 樊尚荣, 等. 临床常见念珠菌对5种抗真菌药物的敏感性[J]. 中华医院感染学杂志, 2005, 15(6):671-674.
LIU X P, LI J W, FAN S R, et al. A etest method for antifungal susceptibility of 173 strains of candidas[J]. Chinese Journal of Nosocomiology, 2005, 15(6):671-674. (in Chinese)
[20] 宋明辉, 崔征, 刘齐歌, 等. 新型隐球菌对氟康唑和两性霉素B的耐药性[J]. 医学研究生学报, 2003, 16(11):880.
SONG M H, CUI Z, LIU Q G, et al. Resistance of Cryptococcus neoformans to fluconazole and amphotericin B[J]. Journal of Medical Postgraduates, 2003, 16(11):880. (in Chinese)
[21] 韩红燕, 钱小毛. 假丝酵母菌属感染的临床分布及耐药性分析[J]. 中华医院感染学杂志, 2010, 20(17):2705-2706.
HAN H Y, QIAN X M. Clinical distribution and drug resistance of Candida spp[J]. Chinese Journal of Nosocomiology, 2010, 20(17):2705-2706. (in Chinese)

Isolation, Identification and Drug Resisitance Analysis of Yeast from Hair of Ailuropoda melanoleuca

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 9

Recommended Articles

Metrics

Comments

[1]	CHENG Xiangning, ZHANG Yun, HE Jiawei, CHEN Zhengkun, JIN Meilan, DING Honglei, WANG Qinghua. Isolation, Identification and Antifungal Susceptibility Analysis of Candida albicans Isolated from Pigeons [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3002-3011.
[2]	CAI Mingyu, ZHANG Hailong, HAI Zhenzhen, QIAO Yarui, DU Jun, ZHOU Xuezhang. The Inflamed Molecular Mechanism Induced by Recombined 14-3-3 Protein of Candida krusei on Bovine Mammary Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1679-1689.
[3]	YU Yan, GU Yu, WANG Chengdong, HU Jing, YANG Aining, YANG Qiao, LI Desheng, ZHANG Hemin, DENG Linhua, LING Shanshan, ZUO Zhicai, PENG Guangneng, ZHONG Zhijun, MA Xiaoping. Dynamic Changes of Dectin-1, TLR-2,TLR-4 and Cytokines in Mice Infected with Microsporum gypseum [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1449-1457.
[4]	YANG Aining, MA Xiaoping, GU Yu, YU Yan, HU Jing, JIANG Yaozhang, PENG Guangneng, ZHONG Zhijun, ZUO Zhicai. The Interaction between Farnesol (Quorum Sensing Molecule) and Candida albicans [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 28-36.
[5]	HAO Baocheng, SONG Xiangdong, GAO Yan, WANG Xuehong, LIU Yu, CHEN Keyuan, LIANG Yan, HU Yuyao, XING Xiaoyong, WEN Fengqin, HU Yonghao, LIANG Jianping. Analysis of the Biosynthesis Pathway of Produced SW by Endophytic Fungi from Locoweed Using Nitrosoguanidine Mutation and High Throughput Sequencing Technology [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 169-182.
[6]	YU Yong-tao, LI Jin-rong, ZHAO Qing-mei, HE Sheng-hu, CHEN Juan, YANG Qi, MAO Yan-ni. Isolation and Identification of Swainsonine-producing Fungi from Astragalus adsurgens Pall [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1770-1780.
[7]	LIU Yue，LIU Yan-wei，BAI Fu-juan，LIU Na，LIU Bo. Identification of Trichophyton mentagrophytes in Rabbit based on the Chitinase Gene Sequence Analysis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1417-1424.
[8]	ZHANG Lei-lei，YU Yong-tao，HE Sheng-hu，ZHAO Qing-mei，GE Song. Influence of Different Factors on Swainsonine Production in Fungal Endophyte from Locoweed [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 163-173.
[9]	YANG Xiao-wen, ZHAO Bao-yu, LU Hao, Bai Ma-sang-mu, CAO Dan-dan, ZHOU Qi-wu. Effect of Different Culture Conditions on Diversity of Endophytic Fungi in Oxytropis kansuensis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1660-1666.