大熊猫西氏贝蛔虫BsABF基因的原核表达及重组蛋白抗菌活性初步分析

doi:10.11843/j.issn.0366-6964.2019.01.018

Abstract

Abstract:

This experiment was conducted to explore the characterization and the antibacterial activity of an antibacterial factor in Baylisascaris schroederi. The gene BsABF was cloned and expressed with prokaryotic expression system. Western blot and immunofluorescence histochemical staining were used to determine the immunological characteristics and the location in the parasite. The antibacterial activity was evaluated by the preliminary antibacterial experiments. Results were as follows:Open reading frame of BsABF gene (276 bp) encoded a 10.01 kDa protein with an N-terminal signal peptide while sharing 94% identity with Ascaris suum. The protein is mainly distributed in hypodermis, intestinal and eggs. The prokaryotic expression of rBsABF had a good immunoreactivity. Meanwhile the antibacterial assessment revealed that the recombinant protein has the antibacterial activity towards Enterococcus faecalis and Escherichia coli in giant pandas. In this study, we have first identified the BsABF gene, and rBsABF does have the antibacterial activity, and it lays the foundation for further study on the functional properties of BsABF in the future.

Key words: giant panda, Baylisascaris schroederi, antimicrobial peptides, immunofluorescence histochemical, antibacterial activity

CLC Number:

S852.731

WU Yiran, GU Xiaobin, XIE Yue, YANG Guangyou. Prokaryotic Expression and Primary Evaluation of Antibacterial Activity of Antibacterial Factor in Baylisascaris schroederi[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 151-158.

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Prokaryotic Expression and Primary Evaluation of Antibacterial Activity of Antibacterial Factor in Baylisascaris schroederi

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