抗菌肽BuforinⅡ衍生物特异性结合金黄色葡萄球菌DNA抑菌机理的研究

doi:10.11843/j.issn.0366-6964.2014.07.018

Abstract

Abstract:

In this paper，the bactericidal mechanism of BF2-A/B，two analogues of antimicrobial peptide BuforinⅡ，specific binding to Staphylococcus aureus genomic DNA had been researched. The results of atomic Force Microscope scan and gel retardation assay showed that both peptides could strongly bind to DNA，and the ability of BF2-B binding to DNA was stronger than that of BF2-A. The results of DNaseⅠ footprinting assay showed that both peptides tended to specifically bind to two domains of S.aureusgenomic DNA，and one of them probably was a fragment of consensus sequence located on the 23S rRNA gene. Furthermore，the results of interference assay to the oxidation phosphorylation of cells suggested that both peptides could obviously inhibit the respiration of S.aureus，inducing a drop in ATP content. Interestingly，BF2-B induced a more remarkable inhibition than BF2-A. The results of researches demonstrated that the powerful antimicrobial activities of both peptides were closely related to penetrate the cytoplasma membrane then specifically bind to two domains of genomic DNA，and inhibit the cell respiration and ATP synthesis. The increase of ability of binding to DNA and inhibiting cell respiration and ATP synthesis explained why BF2-B displayed more excellent antimicrobial activity for S.aureus.

CLC Number:

S852.42

HAO Gang，LE Guo-wei，SHI Yong-hui. The Bactericidal Mechanism of Two Analogues of the Antimicrobial Peptide BuforinⅡSpecific Binding to Staphylococcus aureus Genomic DNA[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1154-1161.

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The Bactericidal Mechanism of Two Analogues of the Antimicrobial Peptide BuforinⅡSpecific Binding to Staphylococcus aureus Genomic DNA

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