猪圆环病毒2型感染PK-15细胞差异表达miRNA和mRNA互作网络

doi:10.11843/j.issn.0366-6964.2019.01.014

Abstract

Abstract:

The purpose of this study was to elucidate changes in miRNA and mRNA expression profiles of PCV2 infected PK-15 cells, and to illuminate the interaction between differentially expressed miRNAs and mRNAs. Stem-loop quantitative PCR was used to detect and identify differential expression levels of multiple hot spot miRNAs in PCV2 infected and uninfected groups. The results of proteomic study of PCV2 infected cells at the 12th hour post infection (hpi) were analyzed (GEO Accession No.:GSE71945) to obtain differentially expressed mRNA and to analyze the interaction between differentially expressed miRNA and mRNA. It was found that miR-10b, miR-128, miR-155-5p, miR-21, miR-29b, miR-361-3p were differentially expressed at 12 hpi. By using miRecords to predict their target genes, 7 105, 9 741, 7 451, 7 183, 7 971 and 8 793 target genes were found, respectively. Totally there were 158 differentially expressed mRNAs that belonged to various cell components and participated in many important biological pathways, and there was a large number of common target genes between the differentially expressed miRNAs candidate target genes and differentially expressed mRNAs. In summary, PCV2 infection can lead to disruption of multiple miRNA expression in cells, resulting in disorganization of miRNA target genes, which in turn influences the normal life events of cells.

Key words: porcine circovirus 2(PCV2), PK-15, miRNA, mRNA, interaction relationship

CLC Number:

S852.659.2

LI Chang, LI Jing, HE Qigai. Integrated Analysis of miRNA and mRNA Network of Porcine Circovirus 2 Infecting Porcine Kidney Cell Line[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 115-125.

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Integrated Analysis of miRNA and mRNA Network of Porcine Circovirus 2 Infecting Porcine Kidney Cell Line

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