miR-200c和miR-429靶向调节绵羊皮下脂肪细胞中SCD1表达的研究

doi:10.11843/j.issn.0366-6964.2019.07.004

Abstract

Abstract: This study aimed to predict and validate the key miRNAs that regulated SCD1 gene expression in ovine subcutaneous adipocytes. miRNAs targeting SCD1 were predicted by bioinformatics softwares of TargetScan, mirDB, StarBase and miRanda. The dual luciferase reporter system was used to verify the target relationships between SCD1 gene and miRNAs. Fluorescent quantitative PCR and Western-blotting techniques were used to detect the effect of miRNAs on SCD1 expression at mRNA and protein levels. The prediction results indicated that SCD1 gene was targeted by both miR-200c and miR-429. Dual luciferase reporter system assay demonstrated that there were target relationships between the 2 miRNAs and SCD1 gene. Fluorescent quantitative PCR analysis showed that miR-200c and miR-429 significantly inhibited SCD1 mRNA expression (P<0.05), and miR-200c was more effective. Western-blotting results showed that miR-200c and miR-429 reduced SCD1 protein level (P<0.01). Microscopical observation showed that miR-200c and miR-429 inhibited ovine subcutaneous lipid droplet formation. In conclusion, miR-200c and miR-429 can down-regulate SCD1 gene expression, thus inhibit ovine lipogenesis.

CLC Number:

S826.2

WANG Shufang, PAN Yangyang, REN Duanyang, ZHAO Yanyan, LI Baojun, QIAO Liying, LIU Wenzhong. Regulation of SCD1 Gene Expression by miR-200c and miR-429 in Ovine Subcutaneous Adipocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1347-1357.

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Regulation of SCD1 Gene Expression by miR-200c and miR-429 in Ovine Subcutaneous Adipocytes

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